PMID: 2502947Aug 15, 1989Paper

Mouse liver phenobarbital-inducible P450 system: purification, characterization, and differential inducibility of four cytochrome P450 isozymes from D2 mouse

Archives of Biochemistry and Biophysics
P Honkakoski, M A Lang

Abstract

Three novel cytochrome P450 isozymes were purified from phenobarbital (PB)-treated D2 mouse liver microsomes and compared to the previously characterized coumarin 7-hydroxylase, P450Coh. The molecular masses were 56.5, 55, 51, and 49.5 kDa, and the peaks of the reduced CO complexes were at 450, 447.5, 451.5, and 449 nm for P450PBI, P450PBII, P450PBIII, and P450Coh, respectively. The NH2-terminal sequences suggest that these isozymes belong to the P450 gene subfamilies 2B, 1A, 2C, and 2A, respectively. On the basis of reconstituted activities and microsomal immunoinhibition studies, P450Coh was the sole catalyst of coumarin 7-hydroxylation. P450PBI was the major isozyme catalyzing the high Km 7-pentoxyresorufin O-dealkylation. This reaction was also mediated at a slower rate by the low Km isozyme, P450PBII. P450PBIII contributed significantly to the microsomal O-deethylation of 7-ethoxyresorufin and N-demethylation of benzphetamine. Western blotting and dot immunobinding analyse of microsomes showed that the induction patterns of the isozymes were different. PB and TCPO-BOP induced all isozymes variably: P450PBI (19- and 31-fold), P450PBII (2- and 3-fold), P450PBIII (9- and 4-fold), and P450Coh (about 2-fold). Pyrazole induced o...Continue Reading

References

Aug 1, 1986·Archives of Biochemistry and Biophysics·S BandieraP E Thomas
Jan 1, 1985·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·P KaipainenM Lang
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R R MeehanM Adesnik
Jan 1, 1987·Annual Review of Biochemistry·D W Nebert, F J Gonzalez
Jan 1, 1986·CRC Critical Reviews in Biochemistry·M Adesnik, M Atchison
Jan 1, 1986·Annual Review of Pharmacology and Toxicology·J P Whitlock
Feb 1, 1987·DNA·D W NebertW Levin
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Oct 1, 1985·European Journal of Biochemistry·R O JuvonenM A Lang
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A Rampersaud, F G Walz

❮ Previous
Next ❯

Citations

Jan 1, 1994·Archives of Toxicology·P PelkonenM Pasanen
Nov 1, 1994·European Journal of Pharmacology·P PellinenM Pasanen
Apr 6, 2000·The Biochemical Journal·P Honkakoski, M Negishi
Mar 1, 1992·British Journal of Clinical Pharmacology·P HonkakoskiO Pelkonen
Feb 1, 1995·British Journal of Pharmacology·T KimonenM Pasanen
Jul 29, 1999·American Journal of Respiratory Cell and Molecular Biology·T SkarinR Toftgârd
Feb 1, 1991·American Journal of Respiratory Cell and Molecular Biology·C H ChichesterC G Plopper
Feb 5, 2000·Environmental Health Perspectives·P PaakkiM Pasanen
Jul 5, 2001·Biochemical and Biophysical Research Communications·R P MeyerB Volk
Oct 9, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Premila AbrahamBanumathi Ramakrishna
Nov 2, 2007·Basic & Clinical Pharmacology & Toxicology·Maria KonstandiMarios Marselos
Aug 22, 2006·European Journal of Clinical Investigation·U S H SimonssonM A Lang
Mar 18, 1995·Chemico-biological Interactions·M LaaksonenO Hänninen
Jan 1, 2000·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·C E CarlbergM Pasanen
Aug 18, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·M KonstandiM Marselos
Feb 14, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H RaunioY Soini
May 1, 1993·Toxicology and Industrial Health·V LongoP G Gervasi
Apr 19, 1996·The Journal of Biological Chemistry·P HonkakoskiM Negishi
Mar 20, 1992·Journal of Chromatography·V Lang
Oct 7, 1994·Biochemical Pharmacology·J MäenpääO Pelkonen
Mar 24, 1993·Biochemical Pharmacology·L M BornheimM A Correia

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.