Mouse mast cell secretory granules can function as intracellular ionic oscillators

Biophysical Journal
I QuesadaP Verdugo

Abstract

Fluorescent Ca2+ probes and digital photo-sectioning techniques were used to directly study the dynamics of Ca2+ in isolated mast cell granules of normal (CB/J) and beige (Bg(j)/Bg(j)) mice. The resting intraluminal free Ca2+ concentration ([Ca2+]L) is 25 +/- 4.2 microM (mean +/- SD, n = 68). Exposure to 3 microM inositol 1,4,5-trisphosphate (InsP3) induced periodic oscillations of luminal Ca2+ ([Ca2+]L) of approximately 10 microM amplitude and a period around 8-10 s. The [Ca2+]L oscillations were accompanied by a corresponding oscillatory release of [Ca2+]L to the extraluminal space. Control experiments using ruthenium red (2 microM) and thapsigargin (100 nM) ruled out artifacts derived from the eventual presence of mitochondria or endoplasmic reticulum in the isolated granule preparation. Oscillations of [Ca2+]L and Ca2+ release result from a Ca2+/K+ exchange process whereby bound Ca is displaced from the heparin polyanionic matrix by inflow of K+ into the granular lumen via an apamin-sensitive Ca2+-sensitive K+ channel (ASK(Ca)), whereas Ca2+ release takes place via an InsP3-receptor-Ca2+ (InsP3-R) channel. These results are consistent with previous observations of [Ca2+]L oscillations and release in/from the endoplasmic ret...Continue Reading

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Citations

Oct 27, 2010·European Biophysics Journal : EBJ·Sumitha NairMiklós Gratzl
Nov 4, 2010·Cellular and Molecular Neurobiology·Seung Hyun YooYong Suk Hur
Nov 23, 2010·Cellular and Molecular Neurobiology·Jaime SantoDomingoJavier Alvarez
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Jan 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Guozhong HuangRoberto Docampo
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