Mouse minipuberty coincides with gonocyte transformation into spermatogonial stem cells: a model for human minipuberty

Reproduction, Fertility, and Development
Ruili LiJohn M Hutson

Abstract

As the transient postnatal hormone surge in humans, known as 'minipuberty', occurs simultaneously with key steps in germ-cell development, we investigated whether similar changes occur in the hypothalamic-pituitary-testicular axis of neonatal mice at a time that would coincide with gonocyte transformation into spermatogonial stem cells (SSC). Serum and testes were collected from C57Bl/6 mice at embryonic Day 17 (E17), birth (postnatal Day 0; P0) and daily until P10. Serum FSH and testosterone levels in both serum and testes were analysed and gene expression of FSH receptor (Fshr), luteinising hormone receptor (Lhr), anti-Müllerian hormone (Amh), octamer-binding transcription factor 4 (Oct-4), membrane type 1 metalloprotease (Mt1-mmp), proto-oncogene C-kit and promyelocytic leukaemia zinc finger (Plzf ) was quantified by real-time polymerase chain reaction. We found a transient surge of serum and testicular testosterone levels between P1 and P3 and a gradual increase in FSH from P1 to P10. Testis Lhr expression remained low from P0 until P10 but Fshr expression peaked between P3 and P6 (P<0.01). The same was found for Oct-4 expression (a gonocyte marker), which surged between P3 and P6 (P<0.01). Mt1-mmp expression peaked at P3 (...Continue Reading

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Citations

Mar 10, 2018·Journal of Neuroendocrinology·C A ToroA Lomniczi
Apr 11, 2020·Endocrinology·Penny A F WhileyKate L Loveland
Sep 8, 2020·Pediatric Surgery International·Emily BurtonRuili Li
Jul 4, 2020·Annals of Pediatric Endocrinology & Metabolism·Christoffer Højrup RenaultAnders Juul
Mar 16, 2021·Journal of Pediatric Surgery·Rosa Maria Vigueras-VillaseñorJulio César Rojas-Castañeda
Apr 27, 2021·Frontiers in Endocrinology·Kai OtsukaAtsushi P Kimura
Jul 2, 2021·Acta histochemica·Alan Jair Contreras-OrtizMaría Elena Trujillo-Ortega
Jul 1, 2020·Hormone Research in Pædiatrics·Marianne Becker, Volker Hesse

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