Abstract
As the transient postnatal hormone surge in humans, known as 'minipuberty', occurs simultaneously with key steps in germ-cell development, we investigated whether similar changes occur in the hypothalamic-pituitary-testicular axis of neonatal mice at a time that would coincide with gonocyte transformation into spermatogonial stem cells (SSC). Serum and testes were collected from C57Bl/6 mice at embryonic Day 17 (E17), birth (postnatal Day 0; P0) and daily until P10. Serum FSH and testosterone levels in both serum and testes were analysed and gene expression of FSH receptor (Fshr), luteinising hormone receptor (Lhr), anti-Müllerian hormone (Amh), octamer-binding transcription factor 4 (Oct-4), membrane type 1 metalloprotease (Mt1-mmp), proto-oncogene C-kit and promyelocytic leukaemia zinc finger (Plzf ) was quantified by real-time polymerase chain reaction. We found a transient surge of serum and testicular testosterone levels between P1 and P3 and a gradual increase in FSH from P1 to P10. Testis Lhr expression remained low from P0 until P10 but Fshr expression peaked between P3 and P6 (P<0.01). The same was found for Oct-4 expression (a gonocyte marker), which surged between P3 and P6 (P<0.01). Mt1-mmp expression peaked at P3 (...Continue Reading
References
Jul 1, 1979·The Journal of Clinical Endocrinology and Metabolism·M G Forest
Oct 1, 1975·Endocrinology·K D Döhler, W Wuttke
Apr 1, 1976·The Journal of Clinical Endocrinology and Metabolism·J S WinterC Faiman
Jan 1, 1987·European Journal of Pediatrics·J C JobP Canlorbe
Feb 1, 1997·Nature Genetics·T R KumarM M Matzuk
Apr 16, 1998·Journal of Pediatric Surgery·T D ClarnetteJ M Hutson
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·A DierichP Sassone-Corsi
Apr 15, 2000·Cells, Tissues, Organs·J M HutsonS Hasthorpe
Oct 13, 2001·Lancet·F Hadziselimovic, B Herzog
May 25, 2004·Nature Genetics·José A CostoyaPier Paolo Pandolfi
Sep 3, 2004·Biology of Reproduction·M JimenezD J Handelsman
Jul 20, 2006·The Journal of Clinical Endocrinology and Metabolism·Ignacio BergadáRodolfo A Rey
Dec 9, 2008·Molecular and Cellular Endocrinology·P J O'ShaughnessyM H Abel
Sep 15, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·D Tim Harwood, David J Handelsman
Feb 11, 2010·The Journal of Steroid Biochemistry and Molecular Biology·K M McNamaraD J Handelsman
Dec 29, 2011·Endocrinology·Matthew C Poling, Alexander S Kauffman
Sep 15, 2012·Journal of Pediatric Surgery·Ruili LiJohn M Hutson
Jan 15, 2013·Frontiers in Endocrinology·John M HutsonDina Cortes
Feb 18, 2014·Journal of Pediatric Surgery·Shu SuRuili Li
Apr 17, 2014·BioMed Research International·D Živković, I Fratrić
Oct 16, 2014·The Journal of Urology·Ruili LiJohn Hutson
Jun 20, 2015·Analytical Chemistry·Pekka Keski-RahkonenDavid J Handelsman
Sep 21, 2015·Journal of Pediatric Surgery·Ruili LiJohn M Hutson
Dec 1, 2015·Journal of Pediatric Surgery·Sarah S K YueRuili Li
Feb 3, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jenny Clarkson, Allan E Herbison
Citations
Mar 10, 2018·Journal of Neuroendocrinology·C A ToroA Lomniczi
Apr 11, 2020·Endocrinology·Penny A F WhileyKate L Loveland
Sep 8, 2020·Pediatric Surgery International·Emily BurtonRuili Li
Jul 4, 2020·Annals of Pediatric Endocrinology & Metabolism·Christoffer Højrup RenaultAnders Juul
Mar 16, 2021·Journal of Pediatric Surgery·Rosa Maria Vigueras-VillaseñorJulio César Rojas-Castañeda
Apr 27, 2021·Frontiers in Endocrinology·Kai OtsukaAtsushi P Kimura
Jul 2, 2021·Acta histochemica·Alan Jair Contreras-OrtizMaría Elena Trujillo-Ortega
Jul 1, 2020·Hormone Research in Pædiatrics·Marianne Becker, Volker Hesse