Mouse Necropsy

Current Protocols in Mouse Biology
Piper M Treuting, Jessica M Snyder

Abstract

Necropsy (also known as autopsy) is the post-mortem dissection of bodies after euthanasia or death and is a scientific examination conducted to observe and dissect the organs, collect tissues, and determine the extent of grossly evident disease. Research necropsies are conducted to obtain specific samples tailored according to study objectives. Diagnostic necropsy may be undertaken when unexpected illness or death occurs. The systematic collection of samples at necropsy is the critical first step in generating morphologic data from animal models. The morphologic (anatomic and histologic) data generates information on changes in cells, tissues, organs, and organ systems providing context for phenotypes (functional and morphological) to the level of the whole organism. Optimal insight into phenotype or pathophysiologic mechanisms is obtained when morphologic data is coupled with laboratory, medical, and molecular findings. This protocol provides a standard for an efficient routine mouse necropsy with brief comments on advanced or alternative techniques.

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Citations

Aug 4, 2018·Nature Protocols·Annabelle C SingerLi-Huei Tsai
May 6, 2019·ILAR Journal·Sue E KnoblaughKrista M D La Perle
Aug 16, 2019·PLoS Pathogens·Kathryn McGuckin WuertzMichael Gale
Nov 2, 2017·Current Protocols in Immunology·Hadar ReichmanAriel Munitz
Jun 20, 2017·Current Protocols in Cell Biology·Alice BusatoRaffaella Mariotti
Sep 12, 2017·Current Protocols in Pharmacology·Matthew C Stubbs, Andrei V Krivtsov
Sep 9, 2017·Current Protocols in Mouse Biology·Brad BolonKelli L Boyd
Aug 27, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Rachid SelmiLilia Messadi

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