Mouse oocyte meiotic resumption and polar body extrusion in vitro are differentially influenced by FSH, epidermal growth factor and meiosis-activating sterol

Human Reproduction
G CoticchioS Cecconi

Abstract

In this study, we compared the relative ability of FSH (100 mIU/ml), epidermal growth factor (EGF) (10 ng/ml), and follicular-fluid meiosis-activating sterol (FF-MAS, 10 micromol/l) to induce meiotic resumption and polar body I (PBI) extrusion in mouse oocytes. Cumulus-enclosed oocytes (CEO) were co-incubated with meiosis-arresting agents, including 4 mmol/l hypoxanthine (Hx), 0.3 mmol/l dibutyryl cAMP (dbcAMP), and 8.5 micromol/l cilostamide, a selective inhibitor of the oocyte-specific phosphodiesterase 3 (PDE 3). In Hx-treated oocytes, FSH, EGF and FF-MAS induced meiosis resumption at very high rates, but only FSH and EGF also promoted PBI extrusion with high frequency. In experiments conducted in the presence of dbcAMP, FF-MAS was unable to promote an increase in germinal vesicle breakdown (GVBD) rate, whereas FSH and EGF generated a response similar to the Hx groups. Neither FSH, EGF nor FF-MAS caused any change in the meiotic status of CEO when meiotic arrest at the germinal vesicle (GV) stage was maintained by cilostamide. In the presence of Hx, naked oocytes (NkO) co-cultured with their cumulus cells were able to respond to the GVBD-inducing effect of FSH and EGF by resuming meiosis at high rate. Collectively, these res...Continue Reading

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Citations

May 1, 2009·The Journal of Reproduction and Development·Hong-Thuy BuiTakashi Miyano
Jul 29, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·D NogueiraJ Smitz
Dec 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Lisa M Mehlmann
Aug 30, 2014·The Journal of Reproduction and Development·Maria Grazia PalmeriniSeverino Antinori
Oct 16, 2015·Histochemistry and Cell Biology·Yi-Bo LuoQing-Yuan Sun
Feb 18, 2010·Reproductive Biomedicine Online·Sandra CecconiGuido Macchiarelli
Jan 10, 2020·Reproduction in Domestic Animals = Zuchthygiene·Felipe Farias Pereira da Câmara BarrosWilter Ricardo Russiano Vicente
Oct 5, 2007·Biology of Reproduction·Fiona H ThomasBarbara C Vanderhyden
Jun 20, 2019·International Journal of Molecular Sciences·Sandra CecconiMauro Maccarrone
Jan 7, 2019·Reproductive Biology and Endocrinology : RB&E·Bo Pan, Julang Li

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