Mouse Ovarian Very Small Embryonic-Like Stem Cells Resist Chemotherapy and Retain Ability to Initiate Oocyte-Specific Differentiation

Reproductive Sciences
Kalpana SriramanSmita Bhutda

Abstract

This study was undertaken to investigate stem cells in adult mouse ovary, the effect of chemotherapy on them and their potential to differentiate into germ cells. Very small embryonic-like stem cells (VSELs) that were SCA-1+/Lin-/CD45-, positive for nuclear octamer-binding transforming factor 4 (OCT-4), Nanog, and cell surface stage-specific embryonic antigen 1, were identified in adult mouse ovary. Chemotherapy resulted in complete loss of follicular reserve and cytoplasmic OCT-4 positive progenitors (ovarian germ stem cells) but VSELs survived. In ovarian surface epithelial (OSE) cell cultures from chemoablated ovary, proliferating germ cell clusters and mouse vasa homolog/growth differentiation factor 9-positive oocyte-like structure were observed by day 6, probably arising as a result of differentiation of the surviving VSELs. Follicle-stimulating hormone (FSH) exerted a direct stimulatory action on the OSE and induced stem cells proliferation and differentiation into premeiotic germ cell clusters during intact chemoablated ovaries culture. The FSH analog pregnant mare serum gonadotropin treatment to chemoablated mice increased the percentage of surviving VSELs in ovary. The results of this study provide evidence for the pr...Continue Reading

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Citations

Mar 27, 2016·Human Reproduction Update·Deepa Bhartiya
Apr 1, 2015·Journal of Ovarian Research·Pranesh GunjalHiren Patel
Nov 19, 2015·Journal of Ovarian Research·Deepa BhartiyaSeema Parte
Sep 11, 2016·Human Reproduction Update·Deepa BhartiyaSreepoorna Unni
Dec 8, 2016·Reproduction in Domestic Animals = Zuchthygiene·G B de SouzaJrv Silva
Mar 30, 2017·Expert Opinion on Biological Therapy·Valeria Stella VanniVeronica Giorgione
Nov 13, 2017·Journal of Assisted Reproduction and Genetics·Deepa Bhartiya, Hiren Patel
Nov 18, 2017·Reproductive Biology and Endocrinology : RB&E·Deepa BhartiyaSeema Parte
Apr 5, 2017·Cellular Reprogramming·Hossein YazdekhastiMehdi Abbasi
Aug 7, 2017·Zygote : the Biology of Gametes and Early Embryos·Tania Janeth Porras-Gómez, Norma Moreno-Mendoza
Oct 22, 2016·Journal of Ovarian Research·Hossein YazdekhastiMehdi Abbasi
Nov 11, 2015·Reproductive Biology and Endocrinology : RB&E·Purna KurkureGanesh Bakshi
Jan 23, 2021·Stem Cell Research & Therapy·Deepa Bhartiya
Jan 26, 2021·Stem Cell Reviews and Reports·Deepa BhartiyaSubhan Ali Mohammad
Dec 25, 2019·Reproductive Toxicology·Laura Silveira AyresElizabeth Obino Cirne-Lima

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Methods Mentioned

BETA
oncotherapy
flow cytometry
PCR
FACS
electrophoresis
flowcytometry

Software Mentioned

Image J
CFX Manager
FACS Diva

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