Mouse preimplantation embryos developed from oocytes injected with round spermatids or spermatozoa have similar but distinct patterns of early messenger RNA expression

Biology of Reproduction
Shotaro HayashiNorman B Hecht

Abstract

Quantitative real-time polymerase chain reaction assay was used to compare the temporal transcriptional activation and mRNA removal for a number of genes in mouse embryos derived by round spermatid injection (ROSI) or intracytoplasmic sperm injection. A number of marker genes with widely different cellular functions were analyzed. Similar patterns of activation were found for the transcription factor Oct 4, the translation initiation factor eukaryotic initiation factor 1A, the L1 ribosomal protein, the chromatin modifying protein histone deacetylase 1, the enzyme hypoxanthine phosphoribosyl transferase, the murine endogenous retrovirus-like element, and the repetitive DNA LINE retrotransposons. Expression of the retrovirus-like mobile element intracisternal A particle, however, was markedly elevated from the two-cell to the blastocyst stages in ROSI embryos. Analyses performed for various paternal mRNAs introduced into the oocyte by the round spermatid, including protamines 1 and 2, transition protein 2, ropporin, and glyceraldehydes 3-phosphate dehydrogenase, revealed all were removed from the preimplantation embryos, albeit with distinct temporal patterns.

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Citations

Sep 2, 2005·Nature Reviews. Genetics·Stephen A Krawetz
Mar 29, 2007·Reproduction, Fertility, and Development·Xiang-Shun Cui, Nam-Hyung Kim
Oct 5, 2010·Human Reproduction·G GiritharanP F Rinaudo
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Oct 30, 2013·PloS One·Peng-Cheng KongMan-Xi Jiang
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May 3, 2007·Molecular Reproduction and Development·Raúl Fernández-GonzalezAlfonso Gutiérrez-Adán
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Jul 1, 2014·European Journal of Immunology·Gero KnittelMatthias Wabl
Oct 5, 2013·Biochimica Et Biophysica Acta·Christina RathkeRenate Renkawitz-Pohl
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Apr 5, 2005·Molecular Reproduction and Development·Yu-Jeong JeongJin Hyun Jun
Jul 16, 2020·Scientific Reports·Won-Ki PangMyung-Geol Pang

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Methods Mentioned

BETA
PCRs
PCR

Software Mentioned

Primer Express

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