PMID: 1212988Oct 1, 1975Paper

Mouse prolactin obtained by pituitary organ culture in a serum-free medium

Endocrinologia Japonica
K Kohmoto

Abstract

Mouse prolactin was purified by organ culture of pituitaries and electrophoresis of the medium. Mouse pituitaries were organ-cultured in 9-cm Petri dishes containing Waymouth's medium (MB 752/1) supplemented with penicillin (50 units/ml), streptomycin (100mug/ml), and bovine insulin (0.12 I.U./ml) for 8 days. Prolactin-rich culture medium was half-saturated with ammonium sulfate and centrifuged. The pellet was subjected to analytical disc electrophoresis (10% acrylamide). Gels were sectioned into 2-mm segments. Prolactin was eluted in 0.04M phosphate buffer (pH 7.2), dialyzed and lyophylized. Two hundred and forty ml medium in which 360 pituitaries were cultured yielded 29.3 mg lyophylized mouse prolactin. Although the preparation contained 2 other bands on acrylamide gel disc electrophoresis, a single precipitin line was seen in immunodiffusion and immunoelectrophoresis, showing the identity of their antigenicity. From these results, two other proteins in the preparation were suggested to be deamidated prolactin.

Citations

Apr 1, 1988·Journal of in Vitro Fertilization and Embryo Transfer : IVF·C MoriT Mori
Feb 1, 1988·Journal of in Vitro Fertilization and Embryo Transfer : IVF·A FukudaK Hoshino
Apr 1, 1989·Journal of in Vitro Fertilization and Embryo Transfer : IVF·A FukudaK Hoshino
Mar 1, 1987·General and Comparative Endocrinology·M KishidaK Shirahata
Mar 1, 1991·General and Comparative Endocrinology·R SuzukiT Hirano
Aug 31, 1982·Biochemistry·T A BewleyF Talamantes
Jan 16, 1984·European Journal of Biochemistry·K KohmotoF Sakiyama
Dec 1, 1983·Molecular and Cellular Endocrinology·H DohiY Shoda
Dec 20, 1978·Biochimica Et Biophysica Acta·L F ShoerF Talamantes
Jan 30, 1979·Biochemical and Biophysical Research Communications·Y N Sinha, S R Baxter
Apr 1, 1978·General and Comparative Endocrinology·M Jibson, F Talamantes

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