PMID: 6407006May 1, 1983Paper

Mouse sn-glycerol-3-phosphate dehydrogenase: molecular cloning and genetic mapping of a cDNA sequence

Proceedings of the National Academy of Sciences of the United States of America
L P Kozak, E H Birkenmeier

Abstract

The isozymes of glycerol-3-phosphate dehydrogenase (GPDH; sn-glycerol-3-phosphate:NAD+2-oxidoreductase, EC 1.1.1.8) in tissues of the mouse are coded for by two structural genes, Gdc-1 and Gdc-2, located on chromosomes 15 and 9, respectively. In order to investigate the expression of these genes, we isolated a GPDH cDNA clone from a mRNA preparation isolated from brown adipose tissue. The GPDH cDNA clone was identified by colony hybridization and hybrid selection of a mRNA that was translated in vitro to produce immunoprecipitable GPDH protein. In blot analysis, the GPDH cDNA hybridized to a single mRNA species that migrated at the position of 23S ribosomal RNA. This GPDH cDNA clone was mapped to the Gdc-1 locus by identification of a restriction enzyme polymorphism present in genomic DNA isolated from Gdc-1 congeneic lines of mice.

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C Labarca, K Paigen
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J Pairault, H Green
Mar 1, 1977·Archives of Biochemistry and Biophysics·J F McGinnis, J De Vellis
Aug 1, 1976·European Journal of Biochemistry·H R Pelham, R J Jackson
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Grunstein, D S Hogness
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Jan 1, 1976·Analytical Biochemistry·J M Bailey, N Davidson
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·L P Kozak
Mar 1, 1981·European Journal of Biochemistry·J S MattickS J Wakil
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J R ParnesJ G Seidman
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·U B RosenbergJ C Perriard
Oct 1, 1981·European Journal of Biochemistry·A R MacLeod

❮ Previous
Next ❯

Citations

Jun 1, 1990·Journal of Cellular Biochemistry·C DaniG Ailhaud
Jan 1, 1991·Mammalian Genome : Official Journal of the International Mammalian Genome Society·E M Eicher, B K Lee
Jan 1, 1991·Mammalian Genome : Official Journal of the International Mammalian Genome Society·K S CheahL Stubbs
Jan 1, 1987·Immunogenetics·P M HogarthE M Eicher
Sep 30, 1985·Biochemical and Biophysical Research Communications·B Bhandari, R E Miller
Sep 1, 1986·Molecular and Cellular Endocrinology·B BhandariR E Miller
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C S BirkenmeierJ E Barker
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E H Birkenmeier, J I Gordon
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C S BirkenmeierJ E Barker
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·M L BloomJ E Barker
Jun 23, 2007·FEBS Letters·Tsutomu KobayashiAnders Jacobsson
Jan 1, 1986·Annals of the New York Academy of Sciences·S BagchiC S Rubin
Jul 1, 1988·Molecular and Cellular Biology·R DuncanM Potter
Feb 1, 1985·Molecular and Cellular Biology·K M Zezulak, H Green

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.