Mouse spermatozoa can synthesize PGE2 and 5-HETE in vitro: stimulatory action of nitric oxide

Prostaglandins, Leukotrienes, and Essential Fatty Acids
M B HerreroM A Gimeno

Abstract

Cauda epididymal spermatozoa were examined for their capacity to synthesize prostaglandins (PGs) and hydroxy acids and the role of nitric oxide (NO) on cyclooxygenase and lipoxygenase enzyme activity was determined. Sperm suspensions were incubated in the presence or absence of 300 microM sodium nitroprusside (NP) and 20 micrograms/ml hemoglobin (Hb) and then incubated with [14C]arachidonic acid (AA) for 60 min. The cyclooxygenase and lipoxygenase products of AA metabolism were measured for their radioactivity. It was found that mouse spermatozoa could synthesize PGE2 and 5-hydroxyeicosatetraenoic acid (5-HETE) under these experimental conditions. The synthesis of these two metabolites was 100% stimulated by NP, the releaser of NO, and the response to NP was completely prevented by Hb, a scavenger of NO. These data provide evidence that mouse spermatozoa can synthesize PGE2 and 5-HETE in the presence of AA in vitro and that NO is involved in the production of AA metabolites in the male gamete.

References

Sep 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M ViggianoM A Gimeno
Dec 1, 1988·European Journal of Obstetrics, Gynecology, and Reproductive Biology·S HayashiT Mori
Jan 1, 1981·Prostaglandins·W SchlegelE Nieschlag
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D SalveminiP Needleman
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·V RettoriS M McCann
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M FranchiM Gimeno
Jul 1, 1994·Journal of Reproduction and Fertility·Y ShalevH Breitbart
Dec 31, 1997·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M B HerreroM A Gimeno

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Citations

Jan 15, 1999·Prostaglandins, Leukotrienes, and Essential Fatty Acids·E GonzálezM Gimeno
Jul 7, 1997·FEBS Letters·M B HerreroM A Gimeno
Nov 25, 1998·Biochemical and Biophysical Research Communications·M B HerreroM F Gimeno

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