Mouse Strain Impacts Fatty Acid Uptake and Trafficking in Liver, Heart, and Brain: A Comparison of C57BL/6 and Swiss Webster Mice

Lipids
D R Seeger, Eric J Murphy

Abstract

C57BL/6 and Swiss Webster mice are used to study lipid metabolism, although differences in fatty acid uptake between these strains have not been reported. Using a steady state kinetic model, [1-(14)C]16:0, [1-(14)C]20:4n-6, or [1-(14)C]22:6n-3 was infused into awake, adult male mice and uptake into liver, heart, and brain determined. The integrated area of [1-(14)C]20:4n-6 in plasma was significantly increased in C57BL/6 mice, but [1-(14)C]16:0 and [1-(14)C]22:6n-3 were not different between groups. In heart, uptake of [1-(14)C]20:4n-6 was increased 1.7-fold in C57BL/6 mice. However, trafficking of [1-(14)C]22:6n-3 into the organic fraction of heart was significantly decreased 33 % in C57BL/6 mice. Although there were limited differences in fatty acid tracer trafficking in liver or brain, [1-(14)C]16:0 incorporation into liver neutral lipids was decreased 18 % in C57BL/6 mice. In heart, the amount of [1-(14)C]16:0 and [1-(14)C]22:6n-3 incorporated into total phospholipids were decreased 45 and 49 %, respectively, in C57BL/6 mice. This was accounted for by a 53 and 37 % decrease in [1-(14)C]16:0 and 44 and 52 % decrease in [1-(14)C]22:6n-3 entering ethanolamine glycerophospholipids and choline glycerophospholipids, respectively....Continue Reading

References

Jun 24, 1989·Molecular and Cellular Biochemistry·J F Glatz, G J van der Vusse
Sep 1, 1988·Diabetes·R S SurwitM N Feinglos
Jan 1, 1988·Neurochemical Research·J M GnaedingerS I Rapoport
Feb 1, 1995·Histochemistry and Cell Biology·W ZschiescheJ F Glatz
Sep 22, 1995·The Journal of Biological Chemistry·B P KennedyW A Cromlish
Mar 10, 1995·The Journal of Biological Chemistry·G LambeauM Lazdunski
Jan 1, 1993·Journal of Neurotrauma·E J Murphy, L A Horrocks
Apr 1, 1996·Journal of Neurochemistry·S C Myers-PayneF Schroeder
Feb 1, 1997·The American Journal of Physiology·J McHowat, S Liu
May 1, 1997·Archives of Biochemistry and Biophysics·C A JollyF Schroeder
Nov 24, 1998·Metabolism: Clinical and Experimental·B L BlackR S Surwit
Nov 18, 2000·Biochimica Et Biophysica Acta·D A Six, E A Dennis
Mar 2, 2002·Journal of Biochemistry·Makoto Murakami, Ichiro Kudo
Aug 22, 2002·Cardiovascular Toxicology·J McHowat, M H Creer
Nov 7, 2002·American Journal of Physiology. Cell Physiology·Sarah A SteerJane McHowat
Jan 9, 2003·Journal of Lipid Research·Thad A RosenbergerStanley I Rapoport
Jun 15, 2004·The Journal of Biological Chemistry·Eric J MurphyJan F C Glatz
Feb 1, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Casey B PatrickEric J Murphy
Oct 29, 2005·Nature Genetics·Ruth ChiaElizabeth M C Fisher
Feb 7, 2008·Chemical Research in Toxicology·Craig E WheelockJohn W Newman
Mar 20, 2008·Lipids·Cameron C MurphyMikhail Y Golovko
Apr 10, 2008·Diabetes·Eric D BerglundDavid H Wasserman
May 19, 2009·Experimental Animals·Kazuyuki MekadaAtsushi Yoshiki
Jul 13, 2011·Journal of Biochemistry·Makoto MurakamiKei Yamamoto
Nov 6, 2012·Neurobiology of Disease·Siew-Na LimAdina T Michael-Titus
Aug 21, 2013·The Journal of Endocrinology·Zhiguo LiuXiaoqiu Xiao

❮ Previous
Next ❯

Citations

Jan 14, 2021·Scientific Reports·Michele CelestinoCarla Mucignat-Caretta
Mar 3, 2020·Molecular and Cellular Endocrinology·Thamiris de SouzaMarta Citelli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.