Mouse↔rat aggregation chimaeras can develop to adulthood

Developmental Biology
Katarzyna BożykAndrzej K Tarkowski

Abstract

In order to examine interactions between cells originating from different species during embryonic development we constructed interspecific mouse↔rat chimaeras by aggregation of 8-cell embryos. Embryos of both species expressed different fluorescent markers (eGFP and DsRed), which enabled us to follow the fate of both components from the moment of aggregation until adulthood. We revealed that in majority of embryos the blastocyst cavity appeared inside the group of rat cells, while the mouse component was allocated to the deeper layer of the inner cell mass and to the polar trophectoderm. However, due to rearrangement of all cells and selective elimination of rat cells, shortly before implantation all primary lineages became chimaeric. Moreover, despite the fact that rat cells were always present in the mural trophectoderm, majority of mouse↔rat chimaeric blastocysts implanted in mouse uterus, and out of those 46% developed into foetuses and pups, half of which were chimaeric. In contrast to mural trophectoderm, polar trophectoderm derivatives, i.e. the placentae of all chimaeras were exclusively of mouse origin. This strongly suggests that the successful postimplantation development of chimaeras is enabled by gradual eliminati...Continue Reading

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Citations

Sep 13, 2019·Biology of Reproduction·Marcin SzpilaAndrzej K Tarkowski
Jul 24, 2020·Development·Berenika Płusa, Anna Piliszek
Feb 16, 2021·World Journal of Stem Cells·Dharmendra KumarWilfried A Kues

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