Mouthwash containing Croton doctoris essential oil: in vitro study using a validated model of caries induction

Future Microbiology
Yasmin Etienne AlbuquerqueFernanda Lourenção Brighenti

Abstract

To validate an in vitro caries model and to evaluate an experimental mouthwash containing Croton doctoris essential oil.  Materials & methods: To validate the experimental model, we used McBain medium and polymicrobial biofilms. The EOM (essential oil mouthwash) was tested using the validated model. Microbial composition (colony-forming unit/ml), acidogenicity, enamel demineralization (percentage of surface enamel hardness loss), cytotoxicity and essential oil composition were evaluated. The model was validated with 0.5% sucrose, duration of 4 days and treatments twice per day. There were statistically significant differences between the EOM, the negative control and chlorhexidine mouthwash in colony-forming unit/ml and percentage of surface enamel hardness loss. Cytotoxicity was similar to that of chlorhexidine mouthwash.  A total of 66.11% of the essential oil consists of sesquiterpenes. The experimental mouthwash showed antimicrobial activity against polymicrobial biofilms and reduced enamel demineralization.

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Citations

Oct 3, 2020·Biofouling·Aline Leite de FariasFernanda Lourenção Brighenti
Dec 23, 2020·Biofouling·Elkin Jahir Florez SalamancaMarlise Inêz Klein
Feb 25, 2021·Future Microbiology·Maria Ac de OliveiraCristiane Y Koga-Ito
Jun 3, 2021·Brazilian Journal of Biology = Revista Brasleira De Biologia·A C S CândidoM T L P Peres
Dec 16, 2021·Biofouling·Aline Leite de FariasFernanda Lourenção Brighenti

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