Movement of vault particles visualized by GFP-tagged major vault protein

Cell and Tissue Research
Marco SlesinaW Volknandt

Abstract

Vaults are abundant large ribonucleoprotein particles. They frequently colocalize with microtubules and accumulate in filamentous actin-rich lamellipodia. To examine the movement of vaults in living cells, a chimera between the green fluorescent protein and the major vault protein was created. This fusion protein assembled into vault particles as assayed by biochemical fractionation and direct observation of living or fixed cells. By fluorescence recovery after photobleaching, we analyzed the bulk transport of vault particles into neuritic tips of PC12 cells treated with nerve growth factor. Confocal laser scanning microscopy demonstrated co-localization of the major vault protein and microtubules. Video microscopy indicated that, whereas the majority of vault particles were stationary, some individual vault particles moved rapidly, consistent with the action of a microtubule-based or actin-based molecular motor.

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Citations

Sep 23, 2008·Nano Letters·Benny C NgSarah H Tolbert
Jun 20, 2007·Photochemistry and Photobiology·Nicholas E DickensonRobert C Dunn
May 11, 2011·The Journal of Biological Chemistry·Hans-Jörg WarnatzMarie-Laure Yaspo
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May 20, 2018·Nature Communications·Felix SigmundGil G Westmeyer
Oct 17, 2020·Cellular and Molecular Life Sciences : CMLS·Jens Claus HahneNicola Valeri

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