Movements and interactions of leukocytes in microgravity

Journal of Biotechnology
M Cogoli-GreuterA Cogoli

Abstract

The mitogenic activation of human lymphocytes resuspended in vitro is dramatically reduced in microgravity. As cell-cell contacts are one of the elements essential for activation, the behaviour of human leukocytes (mainly lymphocytes and monocytes as accessory cells) in the presence of the mitogen concanavalin A was studied in the centrifuge microscope NIZEMI at 0 x g. Aggregates (formed by intercellular bindings of membrane glycoproteins via the tetravalent alpha-glucoside ligand concanavalin A) were found at 0 x g as well as at 1 x g already 12 h after the addition of the mitogen. In general, the aggregates observed at 0 x g after an incubation time of 46 and 78 h were smaller than the corresponding aggregates in the ground control. The findings are of primary importance since they confirm the indirect evidence we had from earlier Spacelab experiments and demonstrate that cell-cell contacts are occurring also in microgravity. In addition, single cells in 0 x g show a significant higher locomotion velocity than the cells at 1 x g. The fact that the locomotion capability is not decreased during the 78-h incubation with concanavalin A provides further evidence that the cells are not proceeding through the cell cycle.

References

Feb 14, 1992·Journal of Immunological Methods·I Newman, P C Wilkinson
Jul 13, 1984·Science·A CogoliP Fuchs-Bislin
Jun 27, 1996·Journal of Biotechnology·P PippiaA Cogoli
Jun 27, 1996·Journal of Biotechnology·P ManieriC Brillouet
Oct 20, 1992·Biotechnology and Bioengineering·B BechlerS B Criswell

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Citations

Feb 24, 2006·European Biophysics Journal : EBJ·Massimo GalimbertiFrancesco S Pavone
Dec 21, 2006·Protoplasma·M A MeloniM Cogoli-Greuter
Jun 27, 1996·Journal of Biotechnology·P PippiaA Cogoli
Mar 9, 2004·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·M Hughes-Fulford
Mar 9, 2004·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·J VassyD Schoevaert
Oct 4, 2002·Nutrition·Andrea T BorchersM Eric Gershwin
Aug 13, 1998·Nature Medicine·B R Unsworth, P I Lelkes
May 23, 2003·Korean Journal of Biological Sciences·A Cogoli
Oct 30, 2008·Cell Communication and Signaling : CCS·Oliver UllrichKerstin Lang
Mar 9, 2006·Radiation and Environmental Biophysics·Lorenzo Manti
May 3, 2001·In Vitro Cellular & Developmental Biology. Animal·P A PlettC M Orschell-Traycoff
Sep 4, 1999·In Vitro Cellular & Developmental Biology. Animal·B Tabourn, L M Spain
Jan 20, 2011·Cytoskeleton·Maria Antonia MeloniMarianne Cogoli-Greuter
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M L LewisP D Bowman
Nov 2, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B B HashemiC F Sams
Aug 20, 2009·Journal of Leukocyte Biology·Nathan GuéguinouJean-Pol Frippiat
Jan 9, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Emily M MartinezMillie Hughes-Fulford
Jul 3, 2021·International Journal of Molecular Sciences·Cora S ThielOliver Ullrich
Jul 21, 2017·NPJ Microgravity·Jean-Pol FrippiatAlexander Choukèr

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