Moving pictures: imaging flow cytometry for drug development

Combinatorial Chemistry & High Throughput Screening
Gary S Elliott

Abstract

As technologies for high throughput and high content screening continue to evolve, new platforms for quantitative cellular imaging will play an increasingly important role in identifying and profiling lead compounds. To gain insight into the effects of a compound on cell morphology or intracellular events, it is necessary to have quality images and the ability to enumerate thousands of data points for statistical relevance. Imaging flow cytometry combines many of the features of flow cytometry, microscopy and imaging as well as a number of unique characteristics. The result is an instrument capable of highly quantitative analysis of cellular behaviors such as receptor internalization, phagocytosis, cell-cell communication, apoptosis and nuclear translocation. This promising new technology and unique type of flow cytometry provides enhanced capabilities for highly multiplexed assays. Here, we review the capabilities of the ImageStream imaging cytometer and discuss several applications relevant to compound screening and profiling.

Citations

Apr 30, 2011·Nature Methods·J Philip McCoy
Aug 19, 2015·Scientific Reports·Yuanyuan Han, Yu-Hwa Lo
Jul 13, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Guohui QiaoJianzhong Wang
May 18, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Hernán E GreccoEli Zamir
Nov 11, 2016·Lab on a Chip·Yuanyuan HanYu-Hwa Lo
Apr 2, 2020·International Journal of Molecular Sciences·Denis V VoroninDaniil N Bratashov
Dec 26, 2018·Expert Opinion on Drug Discovery·Andrea K PomerantzThomas Krucker

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