Abstract
We examined cytokine-stimulated proliferation and survival of human megakaryocyte progenitor cells. We used a reliable, immunoenzymatic method of labeling CD41a-, CD42b-stained megakaryocytes in intact agar cultures to specifically identify and enumerate all megakaryocyte-containing colonies. We examined a previously defined population of cells enriched for megakaryocyte progenitors that coexpress CD34 and the megakaryocyte/platelet marker CD61. These CD34+61+ cells displayed clonogenicity of approximately 30% and contained myeloid, erythroid and megakaryocyte progenitor cells. With single CD34+61+ cells, megakaryocyte growth and development factor ([MGDF], also known as mpl-ligand or thrombopoietin) stimulated 9% of cells to complete their first cell division by day 2 (versus 21% with stem cell factor [SCF], or 13% with interleukin 3 alone). MGDF showed an additive effect with SCF and interleukin 3 to increase this number at least twofold. In purified CD34+61+ cells, MGDF stimulated the survival of megakaryocyte-colony forming cells (CFC) when addition of other proliferative factors was delayed for 5, 10 and 15 days (all p < 0.0001 versus saline control). MGDF also promoted survival of BFU-E and granulocyte-macrophage-CFC for ...Continue Reading
References
Jun 1, 1975·Journal of Cellular Physiology·D Metcalf, H R MacDonald
Nov 1, 1992·British Journal of Cancer·E DeLucaC G Begley
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·I VigonM Souyri
Jan 4, 1990·Nature·G T WilliamsD R Taylor
Jan 1, 1989·International Journal of Cell Cloning·M MisagoS Eto
Jan 1, 1984·Ultrastructural Pathology·V P Collins, U T Brunk
Oct 1, 1981·Journal of Cellular Physiology·G MorstynD Metcalf
Sep 1, 1994·Stem Cells·J L NicholP Hunt
Jan 6, 1995·Science·A C BerardiD T Scadden
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·K KaushanskyC Lofton-Day
May 1, 1995·Stem Cells·E S ChoiJ Chen
May 1, 1995·Stem Cells·C De BruynP Stryckmans
Sep 1, 1995·The Journal of Clinical Investigation·K KaushanskyK H Sprugel
Mar 1, 1993·Stem Cells·R BerthierG Marguerie
Apr 1, 1993·The Journal of Clinical Investigation·K ShimodaY Niho
Sep 10, 1993·Cell·L J FairbairnT M Dexter
Jan 27, 1995·Cell·C J Marshall
Oct 10, 1994·FEBS Letters·Y SohmaH Miyazaki
Jul 1, 1994·Cell·T D BartleyS Swift
Feb 1, 1994·The Histochemical Journal·J L ZhangJ Levin
Jun 16, 1994·Nature·F J de SauvageW J Kuang
Jun 16, 1994·Nature·S LokJ M Kramer
Jun 16, 1994·Nature·F WendlingW Vainchenker
Sep 1, 1993·Stem Cells·G RandallP Jacobs
Sep 1, 1995·Stem Cells·A W RobertsD Metcalf
Oct 1, 1995·Thrombosis Research·C F ToombsB C Varnum
Nov 1, 1995·Stem Cells·P Hunt
Nov 9, 1996·Lancet·R L BasserC G Begley
Citations
Apr 23, 2002·Journal of Cellular Biochemistry·Ying ZhangKatya Ravid
Jun 5, 2001·Mental Retardation and Developmental Disabilities Research Reviews·D BraddockR J Stancliffe
Jan 17, 2002·International Journal of Hematology·R L Basser, C G Begley
Feb 13, 2001·Experimental Hematology·C L ScottC G Begley
Dec 26, 2001·Experimental Hematology·B A AbushullaihN C Daw
Apr 15, 2003·Experimental Hematology·Laurus F SchipperWillem E Fibbe
Jan 21, 2000·Experimental Hematology·T R UlichG Molineux
Jan 25, 2000·Journal of Hematotherapy & Stem Cell Research·L QiuR H Herzig
May 5, 1999·Current Opinion in Hematology·M W Long
Feb 27, 1999·Leukemia & Lymphoma·C Meyer, H G Drexler
May 4, 2001·Growth Factors·R H De BoerJ Cebon
Jul 5, 2005·Expert Opinion on Investigational Drugs·F J Lofts, R Pettengell
Jun 17, 1998·The Journal of Laboratory and Clinical Medicine·C W Jackson
Mar 31, 1999·Baillière's Clinical Haematology·A E von dem BorneM de Haas
Jun 9, 2006·Diagnostic Pathology·Masoud MireskandariKlaus Kayser
Jan 13, 2015·Seminars in Hematology·Allen S Yang
May 3, 2000·British Journal of Haematology·J A Liu YinM Briggs
Jan 1, 1997·Stem Cells·J N IhleK Shimoda
May 26, 1999·Stem Cells·D W van Bekkum
Nov 2, 2002·Blood·David J Kuter, C Glenn Begley
Mar 16, 2002·Blood·Russell L BasserC Glenn Begley
Sep 15, 1998·Acta Paediatrica. Supplement·J L Nichol
Aug 7, 2001·Cancer Investigation·R L Basser, C G Begley
Sep 25, 1999·Growth Factors·W S Alexander
Jun 4, 2021·Drug Design, Development and Therapy·James B BusselJohn W Semple