PMID: 8962685Jan 1, 1995Paper

MPP+ selectively affects calcium homeostasis in mesencephalic cell cultures from embryonal C57/Bl6 mice

Journal of Neural Transmission. General Section
T S ChenW D Rausch

Abstract

1-Methyl-4-phenylpyridinium (MPP+), the active metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) serves as a valuable tool in animal models of Parkinson's disease. Primary cell cultures of mesencephalon from C57/Bl6 mice were used to investigate the effects of various dopaminergic neurotoxins on the intracellular calcium metabolism. MPP+ was compared to its precursor MPTP and a structural analogue paraquat (methylviologen). Direct addition of these neurotoxins (10 microM) to fura-2-labeled cells did not change intracellular calcium concentrations in the presence of 1 mM extracellular calcium. When mesencephalic neurons were exposed to the compounds for 24 hours, only MPP+ led to an increase in calcium concentration in the absence and presence of extracellular calcium (36%, p < 0.05 and 47%, p < 0.01 versus control group). Intracellular calcium concentrations in cortical cultures devoid of dopaminergic cells were not changed by the above neurotoxins. Thus MPP+ is shown to selectively increase intracellular calcium concentrations in mesencephalic cultures.

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Citations

Apr 23, 1999·Journal of Neuroscience Research·G CappellettiR Maci
Jun 22, 2006·Neurotoxicity Research·M GerlachP Riederer
Aug 8, 2008·Molecular Neurobiology·P S VoslerJ Chen
Dec 26, 2009·Neurobiology of Disease·A Raquel EstevesSandra M Cardoso
Sep 2, 2017·Journal of Toxicology and Environmental Health. Part a·Laís Silva FernandesAntonio Cardozo Dos Santos
Oct 14, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jason R RichardsonGary W Miller
Jul 26, 2007·Neural Computation·Alexander S KlyubinChrystopher L Nehaniv
Dec 12, 2012·Journal of Neurochemistry·Stéphanie De SimoniJulie Goemaere

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