MPP3 is recruited to the MPP5 protein scaffold at the retinal outer limiting membrane

The FEBS Journal
Albena KantardzhievaJan Wijnholds

Abstract

Mutations in the human Crumbs homologue 1 (CRB1) gene are a frequent cause of various forms of retinitis pigmentosa. The CRB1-membrane-associated palmitoylated protein (MPP)5 protein complex is thought to organize an intracellular protein scaffold in the retina that is involved in maintenance of photoreceptor-Müller glia cell adhesion. This study focused on the binding characteristics and subcellular localization of MPP3, a novel member of the MPP5 protein scaffold at the outer limiting membrane (OLM), and of the DLG1 protein scaffold at the outer plexiform layer of the retina. MPP3 localized at the photoreceptor synapse and at the subapical region adjacent to adherens junctions at the OLM. Localization studies in human retinae revealed that MPP3 colocalized with MPP5 and CRB1 at the subapical region. MPP3 and MPP4 colocalized with DLG1 at the outer plexiform layer. Mouse Dlg1 formed separate complexes with Mpp3 and Mpp4 in vivo. These data implicate a role for MPP3 in photoreceptor polarity and, by association with MPP5, pinpoint MPP3 as a functional candidate gene for inherited retinopathies. The separate Mpp3/Dlg1 and Mpp4/Dlg1 complexes at the outer plexiform layer point towards additional yet unrecognized functions of thes...Continue Reading

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May 26, 2005·Investigative Ophthalmology & Visual Science·Albena KantardzhievaJan Wijnholds

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Citations

Nov 23, 2011·Journal of Proteome Research·A KantardzhievaW F Sewell
Sep 25, 2012·Human Molecular Genetics·Celso Henrique AlvesJan Wijnholds
Feb 26, 2014·Human Molecular Genetics·Lucie P PellissierJan Wijnholds
Feb 11, 2014·Progress in Retinal and Eye Research·Celso Henrique AlvesJan Wijnholds
Feb 5, 2014·Human Molecular Genetics·Celso Henrique AlvesJan Wijnholds
Apr 15, 2008·Experimental Eye Research·Ilse GosensRonald Roepman
Aug 21, 2007·Glia·Serge A van de PavertJan Wijnholds
Jul 18, 2008·Progress in Retinal and Eye Research·Anneke I den HollanderFrans P M Cremers
Mar 8, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Yasukazu HozumiKaoru Goto
Mar 8, 2006·Human Molecular Genetics·Wendy M AartsenJan Wijnholds
Aug 4, 2006·Human Molecular Genetics·Agnes G S H van RossumJan Wijnholds
Sep 22, 2006·Human Molecular Genetics·Mélisande RichardFrans P M Cremers
Dec 5, 2019·Genes·Peter M J Quinn, Jan Wijnholds
Sep 15, 2020·Frontiers in Neuroscience·Nanda BoonLucie P Pellissier
Oct 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Agnieszka ChytłaAleksander Czogalla

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