Abstract
Astrocytes, the major glial population in the central nervous system (CNS), play an important role in neuronal homeostasis, neurogenesis, and synaptogenesis. The cells have a stellate shape with elaborated processes in the developing CNS. Cultured astrocytes become stellate when the cells undergo differentiation in response to stimuli. Nevertheless, the molecular mechanism for astrocytic stellation is poorly understood. Here, we showed that the addition of serum induced a flat polygonal shape in cultured astrocytes with a reduced level of Mps one binder 2 (Mob2) that is involved in neurite growth by forming stable complex with a nuclear Ser/Thr kinase Dbf2-related protein kinase 1 (NDR1). Furthermore, exposure to a membrane permeable cAMP analogue, dbcAMP, not only induced astrocytic stellation, but also caused an increase in Mob2 expression. Similarly, the upregulation of Mob2 mRNA expression was induced by exposure of astrocytes to pituitary adenylyl cyclase-activating polypeptide (PACAP). Pretreatment with a cAMP/protein kinase A (PKA) inhibitor, KT-5720, significantly blocked the effect of dbcAMP and PACAP on induced upregulation of Mob2 mRNA expression in astrocytes. In addition, the process withdrawal of dbcAMP-treated as...Continue Reading
References
Apr 1, 1992·The Journal of Cell Biology·D M BaortoM L Shelanski
Oct 1, 1990·Brain Research·J E Goldman, B Abramson
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Jan 1, 1996·Brain Research Bulletin·K D RamsellP Cobbett
Dec 26, 1996·Annals of the New York Academy of Sciences·I TatsunoA Arimura
Jan 7, 1998·Trends in Neurosciences·J L RidetF H Gage
Aug 24, 1999·The European Journal of Neuroscience·M Grimaldi, S Cavallaro
Jan 3, 2001·Brain Research·C L Won, Y S Oh
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K M Schmidt-OttM Paul
Sep 8, 2001·Progress in Brain Research·A CastonguayR Robitaille
Dec 19, 2001·Cell·A Colman-LernerR Brent
Apr 26, 2002·Trends in Neurosciences·Craig C GarnerNoam E Ziv
Oct 19, 2002·Science·R Douglas Fields, Beth Stevens-Graham
Dec 31, 2002·Molecular and Cellular Neurosciences·Inmaculada Vallejo, Mario Vallejo
Jan 11, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Olfa MasmoudiMarie-Christine Tonon
Feb 7, 2003·Current Topics in Medicinal Chemistry·Gabriel Fenteany, Shoutian Zhu
Sep 10, 2003·Structure·Elena S StavridiFrancis C Luca
Mar 6, 2004·Journal of Molecular Biology·Luc PonchonAndré Padilla
Jan 19, 2005·The European Journal of Neuroscience·Virgili PerezLaurent Journot
Mar 16, 2005·Cell·Zhi-Chun LaiYing Li
Jun 25, 2005·Molecular Biology of the Cell·Ying HePaul N Adler
Oct 4, 2005·The European Journal of Neuroscience·Mausam GhoshSumantra Das
Oct 22, 2005·Plant Molecular Biology·Sandra CitterioGianni Barcaccia
Apr 12, 2006·Nature Reviews. Molecular Cell Biology·Alexander HergovichBrian A Hemmings
Aug 29, 2006·Journal of Molecular Biology·Serge MrkobradaFrank Sicheri
Nov 9, 2006·Glia·Han-Yun HsiaoShun-Fen Tzeng
Mar 29, 2008·Journal of Neurochemistry·Yu-Peng LiuShun-Fen Tzeng
Jan 6, 2009·Journal of Pharmacological Sciences·Toshiaki KumeAkinori Akaike
Oct 17, 2009·Cell and Tissue Research·Ling-Yu LiuSeng-Sheen Fan
Oct 20, 2009·Current Biology : CB·Alexander HergovichBrian A Hemmings
Nov 17, 2009·Developmental Biology·Li-Lun HoZhi-Chun Lai
Apr 16, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joost SchulteJ Troy Littleton
Jan 18, 2011·FEBS Letters·Cheng-Han LinSeng-Sheen Fan