MPSR1 is a cytoplasmic PQC E3 ligase for eliminating emergent misfolded proteins in Arabidopsis thaliana

Proceedings of the National Academy of Sciences of the United States of America
Jong Hum KimWoo Taek Kim

Abstract

Ubiquitin E3 ligases are crucial for eliminating misfolded proteins before they form cytotoxic aggregates that threaten cell fitness and survival. However, it remains unclear how emerging misfolded proteins in the cytoplasm can be selectively recognized and eliminated by E3 ligases in plants. We found that Misfolded Protein Sensing RING E3 ligase 1 (MPSR1) is an indispensable E3 ligase required for plant survival after protein-damaging stress. Under no stress, MPSR1 is prone to rapid degradation by the 26S proteasome, concealing its protein quality control (PQC) E3 ligase activity. Upon proteotoxic stress, MPSR1 directly senses incipient misfolded proteins and tethers ubiquitins for subsequent degradation. Furthermore, MPSR1 sustains the structural integrity of the proteasome complex at the initial stage of proteotoxic stress. Here, we suggest that the MPSR1 pathway is a constitutive mechanism for proteostasis under protein-damaging stress, as a front-line surveillance system in the cytoplasm.

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Citations

May 16, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ya-Shiuan LaiFederica Brandizzi
Mar 29, 2019·The Plant Journal : for Cell and Molecular Biology·Hye Jo MinWoo Taek Kim
Dec 19, 2019·Plant Signaling & Behavior·Irina LarskayaTatyana Gorshkova
Jun 13, 2018·International Journal of Molecular Sciences·Hayoung SongYoonkang Hur

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Methods Mentioned

BETA
ubiquitination
RNA-seq
transgenic
pulldown
two-hybrid
protein folding
coimmunoprecipitation
immunoprecipitation
gel filtration
size-fractionation

Software Mentioned

LA

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