MR spectroscopy measurement of the diffusion of dimethyl sulfoxide in articular cartilage and comparison to theoretical predictions

Osteoarthritis and Cartilage
A AbazariR B Thompson

Abstract

Cartilage cryopreservation requires optimal loading of protective solutes, most commonly dimethyl sulfoxide (DMSO), to maximize chondrocyte survival. Previously, diffusion models have been used to predict the distribution of solutes in tissue samples, but the accuracy of spatiotemporal predictions of these models have not been validated with empirical studies and remains unknown. In this study, magnetic resonance spectroscopic imaging was used to measure the spatial and temporal changes in DMSO and water concentrations in porcine articular cartilage plugs, throughout 1 h of solute loading. A custom NMR spectroscopic imaging pulse sequence provided water and DMSO concentration images with an in-plane spatial resolution of 135 μm and a temporal resolution of 150 s, repeated for 60 min throughout DMSO loading. Delayed gadolinium-enhanced magnetic resonance of cartilage (d-GEMRIC) imaging provided fixed charge density and spin-density imaging provided water density images prior to DMSO loading. The measured spatial and temporal distribution of DMSO in three different samples was compared to independent predictions of Fick's law and the modified triphasic biomechanical model by Abazari et al. (2011) with the empirical data more clos...Continue Reading

References

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Feb 1, 2013·Biopreservation and Biobanking·Xiaoyi YuShaozhi Zhang

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Citations

Feb 1, 2013·Biopreservation and Biobanking·Xiaoyi YuShaozhi Zhang
Mar 1, 2013·Journal of Biomechanical Engineering·Corinne R HenakJeffrey A Weiss
Mar 19, 2013·Cryobiology·Alireza AbazariLocksley E McGann
Mar 20, 2015·Journal of Biomechanical Engineering·V ArbabiA A Zadpoor
Dec 9, 2016·Journal of Experimental Orthopaedics·Talal TakroniNadr M Jomha
Mar 21, 2021·Npj Regenerative Medicine·Kezhou WuNadr M Jomha

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