MraZ from Escherichia coli: cloning, purification, crystallization and preliminary X-ray analysis

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Melanie A AdamsZongchao Jia

Abstract

The MraZ family of proteins, also referred to as the UPF0040 family, are highly conserved in bacteria and are thought to play a role in cell-wall biosynthesis and cell division. The murein region A (mra) gene cluster encodes MraZ proteins along with a number of other proteins involved in this complex process. To date, there has been no clear functional assignment provided for MraZ proteins and the structure of a homologue from Mycoplasma pneumoniae, MPN314, failed to suggest a molecular function. The b0081 gene from Escherichia coli that encodes the MraZ protein was cloned and the protein was overexpressed, purified and crystallized. This data is presented along with evidence that the E. coli homologue exists in a different oligomeric state to the MPN314 protein.

Citations

Jul 22, 2011·Bioinformatics·Luciana FerrerPeter D Karp
Mar 8, 2016·Biochimie·G Y FisunovV M Govorun
Dec 17, 2009·Journal of Applied Microbiology·G KumarU Sengupta
Mar 25, 2014·Journal of Bacteriology·Jesus M ErasoWilliam Margolin
Aug 5, 2017·Microbiology·Nancy Rivera-GómezErnesto Perez-Rueda

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