MreB polymers and curvature localization are enhanced by RodZ and predict E. coli's cylindrical uniformity

Nature Communications
Benjamin P BrattonRandy M Morgenstein

Abstract

The actin-like protein MreB has been proposed to coordinate the synthesis of the cell wall to determine cell shape in bacteria. MreB is preferentially localized to areas of the cell with specific curved geometries, avoiding the cell poles. It remains unclear whether MreB's curvature preference is regulated by additional factors, and which specific features of MreB promote specific features of rod shape growth. Here, we show that the transmembrane protein RodZ modulates MreB curvature preference and polymer number in E. coli, properties which are regulated independently. An unbiased machine learning analysis shows that MreB polymer number, the total length of MreB polymers, and MreB curvature preference are key correlates of cylindrical uniformity, the variability in radius within a single cell. Changes in the values of these parameters are highly predictive of the resulting changes in cell shape (r2 = 0.93). Our data thus suggest RodZ promotes the assembly of geometrically-localized MreB polymers that lead to the growth of uniform cylinders.

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Citations

Jan 15, 2020·Proceedings of the National Academy of Sciences of the United States of America·David GarenneVincent Noireaux
Feb 12, 2019·Molecular Microbiology·Yusuke YoshiiDaisuke Shiomi
Jan 1, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keisuke KuritaDaisuke Shiomi
Jun 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Huan ZhangBeiyan Nan
May 18, 2020·Biophysical Journal·John N WernerEric A Klein
May 11, 2021·Frontiers in Microbiology·Brody BartonRandy M Morgenstein
Jun 30, 2021·Annual Review of Cell and Developmental Biology·Ethan C Garner
Aug 6, 2021·Annual Review of Microbiology·Patricia D A Rohs, Thomas G Bernhardt

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Methods Mentioned

BETA
dissection

Software Mentioned

MATLAB
LASSO ( Least Absolute Shrinkage and Selection Operator )
LASSO
NIS Elements
SPACECRAFT
LabView

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