Mrf4 determines skeletal muscle identity in Myf5:Myod double-mutant mice

Nature
Lina Kassar-DuchossoyShahragim Tajbakhsh

Abstract

In vertebrates, skeletal muscle is a model for the acquisition of cell fate from stem cells. Two determination factors of the basic helix-loop-helix myogenic regulatory factor (MRF) family, Myf5 and Myod, are thought to direct this transition because double-mutant mice totally lack skeletal muscle fibres and myoblasts. In the absence of these factors, progenitor cells remain multipotent and can change their fate. Gene targeting studies have revealed hierarchical relationships between these and the other MRF genes, Mrf4 and myogenin, where the latter are regarded as differentiation genes. Here we show, using an allelic series of three Myf5 mutants that differentially affect the expression of the genetically linked Mrf4 gene, that skeletal muscle is present in the new Myf5:Myod double-null mice only when Mrf4 expression is not compromised. This finding contradicts the widely held view that myogenic identity is conferred solely by Myf5 and Myod, and identifies Mrf4 as a determination gene. We revise the epistatic relationship of the MRFs, in which both Myf5 and Mrf4 act upstream of Myod to direct embryonic multipotent cells into the myogenic lineage.

References

Dec 31, 1993·Cell·M A RudnickiR Jaenisch
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·C T PhamT J Ley
Feb 13, 1999·Developmental Biology·B KablarM A Rudnicki
Jul 3, 1999·Nature·J L MoranT F Vogt
Jan 15, 2000·Current Topics in Developmental Biology·S Tajbakhsh, M Buckingham
Dec 21, 2002·Science·Jian-Rong LuEric N Olson
Jun 12, 2003·Developmental Biology·Boris KablarMichael A Rudnicki
Jul 31, 2003·Current Opinion in Genetics & Development·Shahragim Tajbakhsh

❮ Previous
Next ❯

Citations

Feb 15, 2011·Cellular and Molecular Life Sciences : CMLS·Shigetoshi Yokoyama, Hiroshi Asahara
Jul 14, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Christopher R Sibley, Matthew J A Wood
May 31, 2012·Histochemistry and Cell Biology·Faisal Yusuf, Beate Brand-Saberi
Feb 10, 2011·Molecular Genetics and Genomics : MGG·Shuang ZhangJudith K Davie
Apr 21, 2011·Biomechanics and Modeling in Mechanobiology·Niamh C NowlanPaula Murphy
Jun 7, 2013·In Vitro Cellular & Developmental Biology. Animal·Satyendra Pal SinghAbhijit Mitra
Sep 8, 2010·Current Oncology Reports·Winston W Huh, Stephen X Skapek
May 11, 2005·Experimental Cell Research·Shahragim Tajbakhsh
Jul 1, 2010·Animal : an International Journal of Animal Bioscience·M BonnetB Picard
Apr 22, 2005·Nature·Frédéric RelaixMargaret Buckingham
Oct 15, 2011·Nature Biotechnology·Thomas Vierbuchen, Marius Wernig
Jun 27, 2006·Nature Cell Biology·Vasily ShininShahragim Tajbakhsh
Jul 19, 2008·Nature Reviews. Genetics·Robert J Bryson-Richardson, Peter D Currie
May 24, 2011·Nature Reviews. Molecular Cell Biology·Thomas Braun, Mathias Gautel
Dec 22, 2011·Nature Reviews. Molecular Cell Biology·Yu Xin Wang, Michael A Rudnicki
Oct 13, 2007·Cell Death and Differentiation·S BiressiG Cossu
May 15, 2009·The Journal of Biological Chemistry·Tetsuaki MiyakeJohn C McDermott
Nov 17, 2010·The Journal of Biological Chemistry·Peter J GianakopoulosIlona S Skerjanc
Dec 26, 2012·The Journal of Biological Chemistry·Anastassia VoronovaIlona S Skerjanc
Aug 5, 2008·The Journal of Biological Chemistry·Anne-Sophie ArmandLeon J De Windt
Oct 7, 2008·The Journal of Biological Chemistry·Kepeng WangZhenguo Wu
Nov 15, 2007·Stem Cells and Development·Sander GrefteJohannes W Von den Hoff
Feb 9, 2007·Molecular Biology of the Cell·Christo ChristovRomain K Gherardi
Jan 26, 2010·Current Opinion in Clinical Nutrition and Metabolic Care·Jong-Sun Kang, Robert S Krauss
Feb 4, 2012·Cold Spring Harbor Perspectives in Biology·C Florian BentzingerMichael A Rudnicki
Sep 19, 2012·Genes & Development·Claire AndersonAnne-Gaëlle Borycki
Jun 21, 2005·Genes & Development·Lina Kassar-DuchossoyShahragim Tajbakhsh
Aug 29, 2009·Development, Growth & Differentiation·Hiroki ShojiFrançoise Poirier
Apr 21, 2010·Molecular and Cellular Biology·Charis L HimedaStephen D Hauschka
Dec 5, 2012·Molecular and Cellular Biology·Mengxi WangMichael P Czech
Dec 10, 2009·Molecular and Cellular Biology·Tetsuaki MiyakeJohn C McDermott
Sep 19, 2007·Molecular and Cellular Biology·Matthew J PotthoffEric N Olson
Jun 15, 2007·Molecular and Cellular Biology·Analeah B HeidtBrian L Black

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.