MRI tracking of bone marrow mesenchymal stem cells labeled with ultra-small superparamagnetic iron oxide nanoparticles in a rat model of temporal lobe epilepsy

Neuroscience Letters
Q LongWeiping Liu

Abstract

Transplantation of bone marrow mesenchymal stem cells (BMSCs) is a promising approach for treatment of epilepsy. To our knowledge, there is little research on magnetic resonance imaging (MRI) tracking of BMSCs labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles in a rat model of temporal lobe epilepsy (TLE). In this study, BMSCs were pre-labeled with USPIO nanoparticles, and then the cell apoptosis, proliferation, surface antigens, and multipotency were investigated. Lithium chloride-pilocarpine induced TLE models were administered by USPIO-labeled BMSCs (U-BMSCs), BMSCs, and saline through lateral ventricle injection as the experimental group, control I group and control II group, respectively, followed by MRI examination, electroencephalography (EEG) and Prussian blue staining. The cell experimental results showed that the labeled USPIO did not affect the biological characteristics and multiple potential of BMSCs. The U-BMSCs can be detected using MRI in vitro and in vivo, and observed in the hippocampus and adjacent parahippocampal cortical areas of the epileptic model. Moreover, electroencephalographic results showed that transplanted U-BMSCs, as well as BMSCs, were capable of reducing the number of e...Continue Reading

References

Mar 1, 1972·Electroencephalography and Clinical Neurophysiology·R J Racine
Jul 17, 2008·Epilepsia·Florian A Siebzehnrubl, Ingmar Blumcke
Apr 1, 2009·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Xu MaisanoJanice R Naegele
Apr 4, 2009·Epilepsy & Behavior : E&B·Margaret P JacobsJohn W Swann
Apr 27, 2010·Annual Review of Biomedical Engineering·Biju Parekkadan, Jack M Milwid
Mar 8, 2011·BMC Neuroscience·Byoung-Kyong MinSeung-Schik Yoo
Mar 15, 2011·Cell Transplantation·Dah-Ching DingShinn-Zong Lin
Jun 29, 2011·Neurological Research·Alireza AbdanipourJavad Mirnajafi-Zadeh
Jul 2, 2011·Life Sciences·Gianina Teribele VenturinJaderson Costa DaCosta
Sep 29, 2011·Neuroscience Letters·Qianfa LongZhou Fei
Oct 31, 2012·Epilepsia·Andreas T Sørensen, Merab Kokaia
Feb 18, 2014·Seizure : the Journal of the British Epilepsy Association·Simone A RomarizBeatriz Monteiro Longo
Apr 15, 2014·International Journal of Nanomedicine·Elizabeth BullAlexander M Seifalian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis