MRL1, a conserved Pentatricopeptide repeat protein, is required for stabilization of rbcL mRNA in Chlamydomonas and Arabidopsis.

The Plant Cell
Xenie JohnsonOlivier Vallon

Abstract

We identify and functionally characterize MRL1, a conserved nuclear-encoded regulator of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. The nonphotosynthetic mrl1 mutant of Chlamydomonas reinhardtii lacks ribulose-1,5-bisphosphate carboxylase/oxygenase, and the resulting block in electron transfer is partially compensated by redirecting electrons toward molecular oxygen via the Mehler reaction. This allows continued electron flow and constitutive nonphotochemical quenching, enhancing cell survival during illumination in spite of photosystem II and photosystem I photoinhibition. The mrl1 mutant transcribes rbcL normally, but the mRNA is unstable. The molecular target of MRL1 is the 5 ' untranslated region of rbcL. MRL1 is located in the chloroplast stroma, in a high molecular mass complex. Treatment with RNase or deletion of the rbcL gene induces a shift of the complex toward lower molecular mass fractions. MRL1 is well conserved throughout the green lineage, much more so than the 10 other pentatricopeptide repeat proteins found in Chlamydomonas. Depending upon the organism, MRL1 contains 11 to 14 pentatricopeptide repeats followed by a novel MRL1-C domain. In Arabidopsis thaliana, MRL1 also acts on rbcL a...Continue Reading

References

Jun 1, 1992·Phytochemistry·G E Edwards, C S Andreo
Aug 23, 1974·Biochimica Et Biophysica Acta·P Joliot, R Delosme
Apr 1, 1993·Molecular and Cellular Biology·D B Stern, K L Kindle
Jan 1, 1993·Methods in Enzymology·J E Boynton, N W Gillham
Jun 12, 1996·Molecular & General Genetics : MGG·N FischerJ D Rochaix
Aug 18, 2000·Biochimie·A Barkan, M Goldschmidt-Clermont
Apr 19, 2002·Current Opinion in Plant Biology·Donald R Ort, Neil R Baker
Sep 14, 2002·Annual Review of Plant Biology·Gilles Peltier, Laurent Cournac
Sep 23, 2003·Methods in Molecular Biology·Jose M Alonso, Anna N Stepanova
Oct 2, 2003·European Journal of Biochemistry·Takahiro NakamuraGadi Schuster
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Krishna K. Niyogi
Apr 1, 2004·The Plant Journal : for Cell and Molecular Biology·Hiroyuki YamazakiToshiharu Shikanai
Sep 7, 2005·Photosynthesis Research·David L Herrin, Jöerg Nickelsen
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R J SpreitzerJ D Rochaix
Jun 21, 2006·Systematic Biology·Maria Anisimova, Olivier Gascuel
Jul 18, 2006·Nucleic Acids Research·Andreas BiegertAndrei N Lupas
Apr 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Katia Wostrikoff, David Stern
May 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Cécile RaynaudYves Choquet
May 31, 2007·Proceedings of the National Academy of Sciences of the United States of America·Richard KurasCatherine de Vitry
Mar 18, 2008·Molecular Biology and Evolution·Nicholas O'TooleIan Small
Aug 30, 2008·BMC Biology·Uwe G MaierMichael Tillich
Nov 14, 2008·Trends in Plant Science·Christian Schmitz-Linneweber, Ian Small

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Citations

Jul 16, 2010·Photosynthesis Research·Benjamin BailleulGiovanni Finazzi
Apr 2, 2013·Photosynthesis Research·Amanda P Cavanagh, David S Kubien
Jul 11, 2013·Photosynthesis Research·James O BerryChristopher M Mure
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sota FujiiIan D Small
Dec 6, 2011·Nucleic Acids Research·Hannes Ruwe, Christian Schmitz-Linneweber
Oct 18, 2011·Eukaryotic Cell·Laurence Meslet-Cladière, Olivier Vallon
Dec 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jana PrikrylAlice Barkan
Jul 24, 2014·Molecular Biology and Evolution·Linnka Lefebvre-LegendreMichel Goldschmidt-Clermont
Dec 18, 2013·Nucleic Acids Research·Karen LoizeauMichel Goldschmidt-Clermont
Jan 30, 2014·Annual Review of Plant Biology·Alice Barkan, Ian Small
Sep 18, 2010·Cellular and Molecular Life Sciences : CMLS·Jessica Jacobs, Ulrich Kück
Jun 1, 2011·The Plant Journal : for Cell and Molecular Biology·Stephan EberhardFrancis-André Wollman
Jun 26, 2012·The Plant Journal : for Cell and Molecular Biology·Christian SchwarzJörg Nickelsen
Apr 23, 2015·The Plant Journal : for Cell and Molecular Biology·Fei WangOlivier Vallon
Sep 12, 2013·RNA Biology·Nicolas J TourasseOlivier Vallon
May 7, 2013·RNA Biology·Mamoru SugitaChieko Sugita
Jun 19, 2013·RNA Biology·Toshiharu Shikanai, Sota Fujii
Apr 28, 2016·The Plant Journal : for Cell and Molecular Biology·Seiya GotoMamoru Sugita
Apr 25, 2007·Veterinary Immunology and Immunopathology·Mads Kjelgaard-HansenStine Jacobsen

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