mRNA 5' ends targeted by cytoplasmic recapping cluster at CAGE tags and select transcripts are alternatively spliced

FEBS Letters
Mikaela R BergerDaniel L Kiss

Abstract

Until cytoplasmic recapping was discovered, decapping was thought to irreversibly destine an mRNA to degradation. Contradicting this idea, we readily observe mRNAs targeted by cytoplasmic capping in uncapped, yet stable forms. 5' rapid amplification of cDNA ends (RACE) shows that nearly all uncapped ends correspond to capped analysis of gene expression tags and that the recapping of ZNF207 mRNA may be restricted to a single splice isoform. Here, a modified RACE approach detected uncapped 5' RNA ends mapping to 46 mRNAs in cells expressing a dominant negative cytoplasmic capping enzyme and in normal cells. Eleven of 46 cloned mRNAs also contained splice isoform-limiting sequences. Collectively, these data reinforce earlier work and suggest that alternative splicing may play a role in targeting transcripts for - and/or determining the position of - cytoplasmic capping.

References

May 20, 2008·Developmental Cell·Brian D GregoryJoseph R Ecker
Jan 27, 2009·Nature·UNKNOWN Affymetrix ENCODE Transcriptome Project, UNKNOWN Cold Spring Harbor Laboratory ENCODE Transcriptome Project
Feb 19, 2009·Molecular and Cellular Biology·Yuichi OtsukaDaniel R Schoenberg
Sep 5, 2009·Trends in Biochemical Sciences·Daniel R Schoenberg, Lynne E Maquat
Apr 15, 2014·Nature Biotechnology·Jessica SeverinAlistair R R Forrest
Nov 8, 2014·Genome Research·Hua-Sheng ChiuPavel Sumazin
Jun 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Anna V IgnatochkinaC Kiong Ho
Dec 18, 2015·Nucleic Acids Research·Daniel L KissDaniel R Schoenberg
Jun 14, 2016·Cell Reports·Cheng-Xu Delon TohYuin-Han Loh
Jun 19, 2016·Nucleic Acids Research·Anand RamanathanSiu-Hong Chan
Jul 19, 2016·Wiley Interdisciplinary Reviews. RNA·Ewa Grudzien-Nogalska, Megerditch Kiledjian
Oct 6, 2017·Nucleic Acids Research·Jackson B TrotmanDaniel R Schoenberg
Nov 23, 2017·Nature Reviews. Molecular Cell Biology·Kathrin LeppekMaria Barna
Sep 1, 2018·The Journal of Biological Chemistry·Jackson B TrotmanDaniel R Schoenberg
Sep 27, 2018·Wiley Interdisciplinary Reviews. RNA·Jackson B Trotman, Daniel R Schoenberg

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Citations

Nov 26, 2020·Open Biology·Bernice A AganaDaniel R Schoenberg
Mar 5, 2021·Bio-protocol·Daniel Del Valle Morales, Daniel R Schoenberg
Oct 19, 2020··Daniel del Valle MoralesDaniel Schoenberg

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