mRNA decay is regulated via sequestration of the conserved 5'-3' exoribonuclease Xrn1 at eisosome in yeast

European Journal of Cell Biology
Katarina VaskovicovaJan Malinsky

Abstract

We describe a novel mechanism of mRNA decay regulation, which takes place under the conditions of glucose deprivation in the yeast Saccharomyces cerevisiae. The regulation is based on temporally stable sequestration of the main 5'-3' mRNA exoribonuclease Xrn1 at the eisosome, a plasma membrane-associated protein complex organizing a specialized membrane microdomain. As documented by monitoring the decay of a specific mRNA substrate in time, Xrn1-mediated mRNA degradation ceases during the accumulation of Xrn1 at eisosome, but the eisosome-associated Xrn1 retains its functionality and can be re-activated when released to cytoplasm following the addition of glucose. In cells lacking the eisosome organizer Pil1, Xrn1 does not associate with the plasma membrane and its activity is preserved till the stationary phase. Thus, properly assembled eisosome is necessary for this kind of Xrn1 regulation, which occurs in a liquid culture as well as in a differentiated colony.

Citations

Jul 28, 2019·Biomolecules·Jakub Zahumensky, Jan Malinsky
Sep 11, 2019·FEMS Microbiology Reviews·Alexandros AthanasopoulosChristos Gournas
May 17, 2018·Oxidative Medicine and Cellular Longevity·Derek WilkinsonZdena Palková
Dec 16, 2017·International Journal of Molecular Sciences·Takeo Wada, Attila Becskei
Sep 18, 2020·Microbiology and Molecular Biology Reviews : MMBR·Carla E LanzeJames B Konopka
Jan 27, 2018·Journal of Fungi·Jenna E FoderaroJames B Konopka
Mar 22, 2018·Proceedings of the National Academy of Sciences of the United States of America·Christos GournasBruno André
Oct 30, 2020·Biomolecules·Katarina VaskovicovaJan Malinsky

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