Abstract
The tongue squamous cell carcinomas (SCCs) are characterized by high mitotic activity, and early detection is desirable. Overexpression of the DNA replication-initiation proteins has been associated with dysplasia and malignancy. Our aim was to determine whether these proteins are useful biomarkers for assessing the development of tongue SCC. We analyzed the mRNA expression of CDC6, CDT1, MCM2 and CDC45 in formalin-fixed, paraffin-embedded benign and malignant tongue tissues using quantitative real-time PCR followed by statistical analysis. We found that the expression levels are significantly higher in malignant SCC than mild precancerous epithelial dysplasia, and the expression levels in general increase with increasing grade of precancerous lesions from mild, moderate to severe epithelial dysplasia. CDC6 and CDC45 expression is dependent of the dysplasia grade and lymph node status. CDT1 expression is higher in severe dysplasia than in mild and moderate dysplasia. MCM2 expression is dependent of the dysplasia grade, lymph node status and clinical stage. The expression of the four genes is independent of tumor size or histological grade. A simple linear regression analysis revealed a linear increase in the mRNA levels of the ...Continue Reading
References
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J F MorrowR B Helling
Oct 17, 1998·Molecular and Cellular Biology·G HateboerK Helin
Oct 28, 1998·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·R T Allison, T Best
Jun 22, 2000·Head & Neck·Y C HendersonG L Clayman
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·U Lehmann, H Kreipe
Feb 19, 2002·Oncogene·Elizabeth ArentsonKyunghee Choi
Mar 2, 2002·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·I KodaniH Ito
Jun 5, 2002·Annual Review of Biochemistry·Stephen P Bell, Anindya Dutta
Jul 12, 2002·Cell·Yuexuan ZhangChun Liang
Jul 26, 2002·CA: a Cancer Journal for Clinicians·Brad W Neville, Terry A Day
Oct 17, 2002·Genes & Development·Seiji Tanaka, John F X Diffley
Oct 24, 2002·Current Opinion in Plant Biology·Crisanto GutierrezJ Carlos del Pozo
Mar 13, 2003·European Journal of Biochemistry·Yukio IshimiSi-Young Song
May 3, 2003·Cancer Cell·Hugh Cam, Brian David Dynlacht
Mar 3, 2004·Oncogene·Kenichi Yoshida, Ituro Inoue
May 20, 2004·Experimental & Molecular Medicine·Jeong-Keun LeeSeong-Doo Hong
Apr 29, 2005·Journal of Clinical Pathology·N MurphyJ J O'Leary
Jul 5, 2007·The FEBS Journal·S PollokF Grosse
Nov 17, 2007·Cancer Research·Michalis LiontosVassilis G Gorgoulis
Oct 13, 2009·Applied and Environmental Microbiology·Jessica C ZweersJan Maarten van Dijl
Citations
Mar 16, 2011·Oral Diseases·M W LingenS-B Woo
Jan 15, 2011·Cancer Science·Yusuke TomitaYasuharu Nishimura
Jun 21, 2012·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Li-Zhong LiangGui-Qing Liao
Feb 27, 2016·Cell Cycle·Carsten KöhlerFrank Grosse
Sep 15, 2012·Technology in Cancer Research & Treatment·Chong-Jin FengJun-Bing Guo
Oct 4, 2017·Oncotarget·Feng DingQiu Zhao
Apr 14, 2018·Frontiers in Molecular Biosciences·Yeon-Soo Seo, Young-Hoon Kang
Aug 24, 2017·Cancer Medicine·Takayuki NakashimaAkira Hara
Jul 19, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Yves Junior SirilHiroyuki Kumamoto
Jan 20, 2016·Oncology Reports·Yubo WangYong He
Aug 8, 2014·Molecular Cancer Research : MCR·Xin ZhangJianxing He
Jul 15, 2021·Pathology Oncology Research : POR·Peng ZhangPing Li
Aug 28, 2021·Life·Hao LinZihao Chen
Sep 18, 2021·Nucleic Acids Research·Kimberlie A WittigChristopher L Sansam