mRNA expression of the DNA replication-initiation proteins in epithelial dysplasia and squamous cell carcinoma of the tongue.

BMC Cancer
Jian-na LiChun Liang

Abstract

The tongue squamous cell carcinomas (SCCs) are characterized by high mitotic activity, and early detection is desirable. Overexpression of the DNA replication-initiation proteins has been associated with dysplasia and malignancy. Our aim was to determine whether these proteins are useful biomarkers for assessing the development of tongue SCC. We analyzed the mRNA expression of CDC6, CDT1, MCM2 and CDC45 in formalin-fixed, paraffin-embedded benign and malignant tongue tissues using quantitative real-time PCR followed by statistical analysis. We found that the expression levels are significantly higher in malignant SCC than mild precancerous epithelial dysplasia, and the expression levels in general increase with increasing grade of precancerous lesions from mild, moderate to severe epithelial dysplasia. CDC6 and CDC45 expression is dependent of the dysplasia grade and lymph node status. CDT1 expression is higher in severe dysplasia than in mild and moderate dysplasia. MCM2 expression is dependent of the dysplasia grade, lymph node status and clinical stage. The expression of the four genes is independent of tumor size or histological grade. A simple linear regression analysis revealed a linear increase in the mRNA levels of the ...Continue Reading

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Citations

Jun 21, 2012·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Li-Zhong LiangGui-Qing Liao
Feb 27, 2016·Cell Cycle·Carsten KöhlerFrank Grosse
Sep 15, 2012·Technology in Cancer Research & Treatment·Chong-Jin FengJun-Bing Guo
Apr 14, 2018·Frontiers in Molecular Biosciences·Yeon-Soo Seo, Young-Hoon Kang
Jul 19, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Yves Junior SirilHiroyuki Kumamoto

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Methods Mentioned

BETA
biopsy
PCR

Software Mentioned

Oligo
SPSS

Related Concepts

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