PMID: 9445054Jan 28, 1998Paper

mRNA instability elements in the human papillomavirus type 16 L2 coding region

Journal of Virology
M SokolowskiS Schwartz

Abstract

Human papillomavirus capsid proteins L1 and L2 are detected only in terminally differentiated cells, indicating that expression of the L1 and L2 genes is blocked in dividing cells. The results presented here establish that the human papillomavirus type 16 L2 coding region contains cis-acting inhibitory sequences. When placed downstream of a reporter gene, the human papillomavirus type 16 L2 sequence reduced both mRNA and protein levels in an orientation-dependent manner. Deletion analysis revealed that the L2 sequence contains two cis-acting inhibitory RNA regions. We identified an inhibitory region in the 5'-most 845 nucleotides of L2 that acted by reducing cytoplasmic mRNA stability and a second, weaker inhibitory region in the 3' end of L2. In contrast, human papillomavirus type 1 L1 and L2 genes did not encode strong inhibitory sequences. This result is consistent with observations of high virus production in human papillomavirus type 1-infected tissue, whereas only low levels of human papillomavirus type 16 virions are detectable in infected epithelium. The presence of inhibitory sequences in the L1 and L2 mRNAs may aid the virus in avoiding the host immunosurveillance and in establishing persistent infections.

References

Jul 1, 1991·Journal of Virology·P F Lambert
Nov 22, 1991·Science·H zur Hausen
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D M KoellerJ B Harford
Aug 1, 1985·Virology·K SeedorfW G Röwekamp
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Oct 17, 1985·Nature·J M CaronM W Kirschner
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Aug 1, 1981·Cell·D W ClevelandM W Kirschner
Sep 1, 1995·Microbiological Reviews·J Ross
Aug 13, 1993·Cell·A B Sachs
Apr 21, 1995·Cell·C A Beelman, R Parker
Jan 1, 1994·Annual Review of Microbiology·H zur Hausen, E M de Villiers
Jan 1, 1994·Intervirology·L T Chow, T R Broker
May 1, 1994·Antiviral Research·M A Stanley
Aug 1, 1993·Molecular and Cellular Biology·C L WellingtonJ G Belasco
Nov 1, 1995·Trends in Biochemical Sciences·C Y Chen, A B Shyu
Jul 1, 1996·Molecular and Cellular Biology·N M YeildingW M Lee
Jul 1, 1996·Molecular and Cellular Biology·A N Hennigan, A Jacobson

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Citations

Oct 3, 1999·AIDS Research and Human Retroviruses·M H NaghaviA Vahlne
Aug 8, 2001·The Journal of Biological Chemistry·C A DavisH S Nick
May 17, 2006·PLoS Biology·Grzegorz KudlaMaciej Zylicz
Dec 9, 2009·Nucleic Acids Research·Xianjun DongBoris Lenhard
Nov 26, 1999·The Journal of General Virology·M WagnerM Renner
May 8, 2015·Biomolecules·Naoko Kajitani, Stefan Schwartz
Apr 4, 2006·Molecular and Cellular Biochemistry·Seiichiro MoriTadahito Kanda
Oct 18, 2005·BMC Infectious Diseases·Jeppe VintherBodil Norrild
Dec 25, 2003·Journal of Virology·Christopher B BuckJohn T Schiller
Nov 26, 2010·Nature Reviews. Genetics·Joshua B Plotkin, Grzegorz Kudla
Mar 12, 2013·Nature Reviews. Microbiology·Cecilia Johansson, Stefan Schwartz
Jan 31, 2015·Nucleic Acids Research·Dawid GłówJanusz M Bujnicki
Mar 26, 2002·Molecular and Cellular Endocrinology·R S SellersThomas J Rosol
Sep 2, 2009·Virus Research·Ulrike SiemetzkiW Ian Lipkin

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