PMID: 7527263Aug 1, 1994Paper

mRNA levels and methylation patterns of the 2-5A synthetase gene in control and Alzheimer's disease (AD) fibroblasts

Biochemistry and Molecular Biology International
S J AnJ M Wu

Abstract

We have examined the mRNA levels and methylation patterns of the interferon-inducible 2',5'-oligoadenylate (2-5A) synthetase gene in skin fibroblasts derived from AD and age/sex-matched control subjects. Northern or slot hybridization analysis of total RNA showed a 63% and 46% decrease in the steady state level of 2-5A synthetase mRNA in AD cells as compared to controls, following a 24 h and 48 h treatment with IFN-beta ser. The 2-5A synthetase gene as studied by Southern analysis using the methylation-sensitive restriction enzymes HpaII and Msp I was found to be hypomethylated in AD cells. No difference in methylation patterns of the actin gene existed between control and AD fibroblasts.

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