PMID: 9545106Apr 17, 1998Paper

mRNA levels of membrane-type 1 matrix metalloproteinase (MT1-MMP), MMP-2, and MMP-9 and of their inhibitors TIMP-2 and TIMP-3 in normal thyrocytes and thyroid carcinoma cell lines

Thyroid : Official Journal of the American Thyroid Association
A HofmannG Aust

Abstract

Thyroid cancer can degrade basement membranes and invade tissues. This depends on a cascade of matrix metalloproteinases involving membrane-type 1 matrix metalloproteinase (MT1-MMP), MMP-2, and MMP-9. We analyzed the expression and role of these MMPs and their specific inhibitors TIMP-2 and TIMP-3 in human highly purified thyroid epithelial, C 643, HTh 74, SW 1736, and 8505 C thyroid carcinoma and thyroid-derived fibroblast cell cultures. The effect of phorbol-myristate acetate (PMA), and of the inflammatory cytokines interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha) on MMP and TIMP mRNA levels were monitored by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) including an internal homologous competitor fragment. The highest MT1-MMP mRNA levels were found in thyroid-derived fibroblasts. The MT1-MMP mRNA expression was increased up to 10-fold by PMA, while all other growth factors tested had only negligible effects. The thyroid carcinoma cells themselves did not seem to play a crucial role in the production of MT1-MMP in thyroid tumors. Higher MMP-2 mRNA levels were found in all cell types investigated. The highest MMP-2 mRNA levels were determined in thyroid-derived fibroblasts and HTh 74...Continue Reading

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Citations

Sep 23, 2000·Molecular and Cellular Endocrinology·M Páez PeredaG K Stalla
Jul 13, 2000·International Journal of Radiation Oncology, Biology, Physics·T E MerchantB N Rao
Mar 13, 2003·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Y-H ParkC K Min
Sep 13, 2002·Thyroid : Official Journal of the American Thyroid Association·Jan KomorowskiH Stepień
Feb 24, 2001·Thyroid : Official Journal of the American Thyroid Association·Z Kraiem, S Korem
Jan 27, 2005·Thyroid : Official Journal of the American Thyroid Association·E BaldiniM D'Armiento
Jul 21, 2005·Thyroid : Official Journal of the American Thyroid Association·Jamie C Mitchell, Sareh Parangi
Jul 25, 2008·Thyroid : Official Journal of the American Thyroid Association·Beatriz G CavalheiroLenine G Brandão
Dec 24, 2002·American Journal of Respiratory Cell and Molecular Biology·Jeffrey J Atkinson, Robert M Senior
Feb 18, 2005·American Journal of Clinical Pathology·Ben DavidsonFahri Saatcioglu
May 31, 2002·Journal of Endocrinological Investigation·A PatelG L Francis
Oct 31, 2006·The American Journal of Pathology·Joerg GalleGabriela Aust
Oct 23, 2003·Endocrine Reviews·Helen E TurnerJohn A H Wass

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