mRNA m6 A plays opposite role in regulating UCP2 and PNPLA2 protein expression in adipocytes

International Journal of Obesity : Journal of the International Association for the Study of Obesity
Xinxia WangYizhen Wang

Abstract

N6-methyladenosine (m6A) modification of mRNA plays an important role in regulating adipogenesis. However, its underlying mechanism remains largely unknown. Using Jinhua and Landrace pigs as fat and lean models, we presented a comprehensive transcriptome-wide m6A profiling in adipose tissues from these two pig breeds. Two differentially methylated genes were selected to explore the mechanisms of m6A-mediated regulation of gene function. The ratio of m6A/A in the layer of backfat (LB) was significantly higher in Landrace than that in Jinhua. Transcriptome-wide m6A profiling revealed that m6A modification on mRNA occurs in the conserved sequence motif of RRACH and that the pig transcriptome contains 0.53-0.91 peak per actively expressed transcript. The relative density of m6A peaks in the 3'UTR were higher than in 5'UTR. Genes with common m6A peaks from both Landrace (L-LB) and Jinhua (J-LB) were enriched in RNA splicing and cellular lipid metabolic process. The unique m6A peak genes (UMGs) from L-LB were mainly enriched in the extracellular matrix (ECM) and collagen catabolic process, whereas the UMGs from J-LB are mainly involved in RNA splicing, etc. Lipid metabolism processes were not significantly enriched in the UMGs from L...Continue Reading

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Citations

Jan 16, 2019·Journal of Cellular Physiology·Zhen BiXinxia Wang
Oct 20, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qin JiangXinxia Wang
Mar 14, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yongxi YaoXinxia Wang
Sep 17, 2020·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Ruifan Wu, Xinxia Wang
Jan 31, 2020·Frontiers in Endocrinology·Xuejiao ChenGuowei Li
May 24, 2020·Nature Communications·Zihan LiHongsheng Wang
Apr 12, 2020·Journal of Hematology & Oncology·Yanchun ZhaoWanzhuo Xie
Aug 18, 2018·Mediators of Inflammation·Mirian SamblasFermín Milagro
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Feb 26, 2021·Journal of Cardiovascular Translational Research·Ye-Shi ChenKai Yin
Feb 23, 2021·Frontiers in Cell and Developmental Biology·Bohan ChengShouzhi Wang
May 13, 2021·Journal of Diabetes Investigation·Mingli Sun, Xinan Zhang
Dec 25, 2021·Frontiers in Endocrinology·Torunn RønningenYvonne Böttcher

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Methods Mentioned

BETA
immunoprecipitation
PCR
Protein Assay
electrophoresis
RIP
PAR-CLIP

Software Mentioned

ImageJ
Cufflink
SPSS
Ensemble
Tophat
HOMER

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