mRNA maturation in giant viruses: variation on a theme.

Nucleic Acids Research
Stéphane PrietChantal Abergel

Abstract

Giant viruses from the Mimiviridae family replicate entirely in their host cytoplasm where their genes are transcribed by a viral transcription apparatus. mRNA polyadenylation uniquely occurs at hairpin-forming palindromic sequences terminating viral transcripts. Here we show that a conserved gene cluster both encode the enzyme responsible for the hairpin cleavage and the viral polyA polymerases (vPAP). Unexpectedly, the vPAPs are homodimeric and uniquely self-processive. The vPAP backbone structures exhibit a symmetrical architecture with two subdomains sharing a nucleotidyltransferase topology, suggesting that vPAPs originate from an ancestral duplication. A Poxvirus processivity factor homologue encoded by Megavirus chilensis displays a conserved 5'-GpppA 2'O methyltransferase activity but is also able to internally methylate the mRNAs' polyA tails. These findings elucidate how the arm wrestling between hosts and their viruses to access the translation machinery is taking place in Mimiviridae.

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B S SchnierleB Moss
Feb 1, 1980·Virology·J KuznarE Viñuela
Feb 1, 1980·Journal of Neurochemistry·H Yamamoto, Y Takahashi
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·K S Wilson, P H von Hippel
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A BakerJ A McCammon
Nov 2, 2001·Journal of Virology·L M IyerE V Koonin
Sep 6, 2002·Biochimica Et Biophysica Acta·Richard C Condit, Edward G Niles
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Aug 15, 2003·The Journal of General Virology·Steven S Broyles
Oct 24, 2003·Acta Crystallographica. Section D, Biological Crystallography·G BricogneW Paciorek
Jul 24, 2004·Acta Crystallographica. Section D, Biological Crystallography·Chantal Abergel
Oct 16, 2004·Science·Didier RaoultJean-Michel Claverie
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·E BlancG Bricogne
Oct 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Karsten SuhreJean-Michel Claverie
Sep 8, 2006·Journal of Bioenergetics and Biomembranes·Sandra JeudyChantal Abergel
Sep 15, 2006·Journal of Virology·Patricia RenestoJean-Michel Claverie
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Sep 18, 2007·RNA·Georges Martin, Walter Keller
Oct 18, 2008·Nature Reviews. Genetics·William F MarzluffRobert J Duronio
Feb 17, 2009·RNA·Delphine BenarrochStewart Shuman
May 1, 2009·Genome Research·Deborah ByrneChantal Abergel
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Aug 6, 2009·Annual Review of Genetics·Jean-Michel Claverie, Chantal Abergel
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yael MutsafiAbraham Minsky
Apr 3, 2010·Genome Research·Matthieu LegendreJean-Michel Claverie
Apr 28, 2010·PLoS Pathogens·Mickaël BouvetEtienne Decroly
May 12, 2010·Nucleic Acids Research·Liisa Holm, Päivi Rosenström
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Matthias G FischerCurtis A Suttle
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sheree YauRicardo Cavicchioli
Oct 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Defne ArslanJean-Michel Claverie
Oct 18, 2011·Nature Reviews. Microbiology·Derek Walsh, Ian Mohr
Apr 7, 2012·Communicative & Integrative Biology·Matthieu LegendreJean-Michel Claverie

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Citations

Sep 24, 2015·FEMS Microbiology Reviews·Chantal AbergelJean-Michel Claverie
Apr 22, 2017·Nature Communications·Elisabeth FabreChantal Abergel
May 2, 2020·Médecine sciences : M/S·Manon Dassa-ValzerAmel Latifi
Dec 3, 2016·The ISME Journal·Rachel Ashley LevinMadeleine Josephine Henriette van Oppen

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Methods Mentioned

BETA
biolayer interferometry
biosensors
PISA
PCR
Gel filtration

Software Mentioned

Gauge
DALI
Mfold
AutoBUSTER
Phaser
PISA
Image
PSI
autoSHARP
BLAST

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