PMID: 2500423Jul 1, 1989Paper

mRNA secondary structure in an open reading frame reduces translation efficiency in Bacillus subtilis

Journal of Bacteriology
M Kubo, T Imanaka

Abstract

The structural gene for thermostable neutral protease, nprM, has only one stacking region, whose energy is -16.3 kcal/mol (-68.2 kJ/mol). Mutations for increasing (-30.8 kcal/mol [128.9 kJ/mol] and decreasing (-5.0 kcal/mol [-20.9 kJ/mol]) the energy of the stacking region were introduced in nprM on the recombinant plasmid pMK1 by using site-directed mutagenesis without any amino acid substitutions. The resultant plasmids were designated pMK2 and pMK3, respectively. The enzyme productivity of the pMK2 carrier was about 40% lower than that of pMK1, whereas the productivity of the pMK3 carrier was about 5% higher. The higher the stability of the stacking regions, the lower the enzyme productivity that was observed. mRNA concentrations were almost the same in the cells harboring these three plasmids. These results indicate that the secondary structure of mRNA reduces the translation efficiency.

References

May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H C Wong, S Chang
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Citations

Jan 1, 1990·Annals of the New York Academy of Sciences·W B MacklinZ O Obeso
Aug 6, 2008·Applied Biochemistry and Biotechnology·Dasari Venkata Krishna RaoAdibhatla Kali Satya Bhujanga Rao
May 14, 2011·Biotechnology Journal·Evelina Angov
Jul 24, 2014·Mobile Genetic Elements·Lixin DaiJef D Boeke
Apr 27, 2013·Journal of the Science of Food and Agriculture·Min-Su KimYoung-Wan Kim
May 1, 2021·Genes·Sundaramoorthy SrinivasanLluís Ribas de Pouplana
Sep 1, 1991·Research in Microbiology·B DiderichsenP L Jørgensen

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