mRNA sequences for Haemonchus contortus intestinal cathepsin B-like cysteine proteases display an extreme in abundance and diversity compared with other adult mammalian parasitic nematodes

Molecular and Biochemical Parasitology
Douglas P JasmerJames P McCarter

Abstract

Cathepsin B-like cysteine protease (cbl) genes produce the most abundant mRNAs ( approximately 16%) detected in the adult female intestine of the parasitic nematode Haemonchus contortus. CBL enzymes appear to digest host proteins and are vaccine candidates for immune control of H. contortus and potentially other parasitic nematodes. Hence, it is important to quantify the extent of diversity of H. contortuscbl genes. Here, expressed sequence tags (ESTs) were used to assess both the size and diversity of the H. contortuscbl gene family. Contig analysis of 686 cbl ESTs from a USA isolate resolved 123 clusters. ESTs were grouped into discrete sets and analyzed using an additive model. Discovery of new cbl clusters increased with each set and reached a terminal rate of about 1 per 10 ESTs. The extreme diversity was unique to cbls relative to other genes investigated and was ascribed to specific cbl clades. Sixty percent of cbl clusters from a UK isolate were shared with those identified in the USA isolate, suggesting conservation of cbl gene repertoires across regions, although minor to moderate geographic variation cannot be excluded. Sequence comparisons also suggested high potential for antigenic diversity among CBL proteins, whi...Continue Reading

References

Nov 1, 1992·Molecular and Biochemical Parasitology·D PrattG N Cox
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L H HolleyM Karplus
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1985·Developmental Biology·E M Hedgecock, J G White
Feb 1, 1995·Current Opinion in Immunology·H G Rammensee
Oct 18, 2000·The Journal of Biological Chemistry·S Shompole, D P Jasmer
Nov 12, 2002·The EMBO Journal·Leath A TonkinBrenda L Bass
Mar 8, 2003·Parasitology·R M Maizels, A Kurniawan-Atmadja
Mar 28, 2003·Acta Tropica·John E Donelson
Sep 6, 2003·Trends in Parasitology·Angela L WilliamsonAlex Loukas

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Citations

May 15, 2013·Trends in Parasitology·Daniel SojkaPetr Kopáček
Apr 28, 2005·BMC Genomics·Makedonka MitrevaRichard K Wilson
Jul 4, 2008·PLoS Neglected Tropical Diseases·James WasmuthMark Blaxter
Jul 18, 2012·PLoS Neglected Tropical Diseases·Jon J VermeireConor R Caffrey
Jun 15, 2007·Veterinary Parasitology·Makedonka MitrevaDouglas P Jasmer
Feb 3, 2007·Parasite Immunology·D P JasmerW C Brown
May 27, 2008·Molecular and Biochemical Parasitology·Najju RanjitAlex Loukas
Sep 23, 2014·Molecular and Biochemical Parasitology·I M AllaieM Sankar
Nov 23, 2006·Developmental Biology·James D McGheeA Gordon Robertson
Jan 9, 2015·PLoS Neglected Tropical Diseases·Douglas P JasmerMakedonka Mitreva
Jan 23, 2015·Molecular & Cellular Proteomics : MCP·Bruce A RosaMakedonka Mitreva
Aug 24, 2018·PLoS Neglected Tropical Diseases·Conor R CaffreyDavid Smith
Jul 28, 2006·Journal of Veterinary Science·Charles I MulekeLi Xiangrui
Apr 25, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Charles I MulekeLi Xiangrui
Aug 14, 2019·Frontiers in Genetics·Douglas P JasmerMakedonka Mitreva

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