mRNA transcription of prostaglandin synthases and their products in the equine endometrium in the course of fibrosis

Theriogenology
A SzóstekD J Skarzynski

Abstract

Accurate regulation of the reproductive cycle and successful implantation depend on proper functioning of the endometrium. The aim of this study was to determine whether mRNA transcription of specific enzymes responsible for prostaglandin (PG) synthesis (prostaglandin-endoperoxide synthase, PTGS-2; prostaglandin F(2α) synthase, PGFS; and prostaglandin E(2) synthases, PGES) and PG concentrations in endometrial extracts would change in moderate (Kenney's Category II) and severe phases of fibrosis (Kenney's Category III; endometrosis), compared with healthy endometrium (Kenney's Category I), during the estrous cycle. Endometrial tissues samples were obtained from mares at the early (n = 12), mid (n = 12) and late (n = 12) luteal phases and the follicular phase (n = 12) of the estrous cycle. Additionally, all endometria were classified microscopically as belonging to Categories I and II or III according to the Kenney classification, resulting in allocation of 4 samples for each subcategory, e.g., mid luteal I, II and III. Relative mRNA transcription was quantified using Real-time PCR. Concentrations of PGE(2) and PGF(2α) in the endometrial extracts were determined using enzyme-linked immunosorbent assay (EIA). In Category I, PTGS-2...Continue Reading

References

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Citations

Feb 1, 2014·The Journal of Endocrinology·Anna Z SzóstekDariusz J Skarzynski
Oct 4, 2014·Reproduction in Domestic Animals = Zuchthygiene·M R RebordãoG Ferreira-Dias
Aug 16, 2013·Biology of Reproduction·Anna Z SzóstekDariusz J Skarzynski
Oct 8, 2014·Polish Journal of Veterinary Sciences·J BuczkowskaJ Mrowiec
Jan 26, 2020·Scientific Reports·Anna Szóstek-MioduchowskaKiyoshi Okuda
Oct 8, 2020·Stem Cell Reviews and Reports·Joanna BukowskaBarbara Gawrońska-Kozak
May 23, 2020·Theriogenology·Anna Zuzanna Szóstek-MioduchowskaDariusz Jan Skarzynski
Jan 25, 2020·Theriogenology·Dariusz J SkarzynskiGraça M Ferreira-Dias

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