MS26/CYP704B is required for anther and pollen wall development in bread wheat (Triticum aestivum L.) and combining mutations in all three homeologs causes male sterility

PloS One
Manjit SinghA Mark Cigan

Abstract

Development of anthers and pollen represents an important aspect of the life cycle in flowering plants. Genes contributing to anther and pollen development have been widely studied in many plant species. Ms26/CYP704B genes play an important role in pollen development through biosynthesis of sporopollenin for pollen exine formation. To investigate the role of Ms26/CYP704B genes in anther and pollen development of bread wheat, mutations in the A-, B-, and D-homeologs of the putative Ms26/CYP704B gene were analyzed. Single and double homozygous mutants in any of the homeologs did not affect pollen development and male fertility. Triple homozygous mutants resulted in completely male sterile plants that were defective in pollen and anther development. Additionally, double homozygous-single heterozygous mutants were also male sterile although with varying levels of residual fertility. The fertility of these triple mutants was dependent upon the homeolog contributing the wild-type allele. Two heterologous Ms26/CYP704B genes, when transformed into a triple homozygous mutant background, completely restored male fertility, whereas a single gene was unable to restore fertility. Functional analysis of Ms26/CYP704B furthers the understandin...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 19, 2000·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·F AhlersR Wiermann
Feb 15, 2005·Plant Physiology·Hui Duan, Mary A Schuler
Oct 9, 2009·The Plant Journal : for Cell and Molecular Biology·Huirong GaoL Alexander Lyznik
Sep 21, 2010·BMC Genomics·Alina R AkhunovaEduard D Akhunov
Oct 12, 2010·Plant Signaling & Behavior·Hui Li, Dabing Zhang
Feb 1, 2011·Annual Review of Plant Biology·Tohru Ariizumi, Kinya Toriyama
Feb 4, 2012·The Arabidopsis Book·Søren BakDanièle Werck-Reichhart
Aug 4, 2012·Journal of Experimental Botany·Moshe FeldmanAbraham Korol
Jun 12, 2013·Cytogenetic and Genome Research·A Madlung, J F Wendel
Jul 31, 2013·Plant Physiology·Trevor H Yeats, Jocelyn K C Rose
Oct 12, 2013·The Plant Journal : for Cell and Molecular Biology·Vesna DjukanovicLeszek Alexander Lyznik
May 7, 2014·Journal of Integrative Plant Biology·Xijia YangDabing Zhang
Jun 8, 2014·Phytochemistry·Teagen D QuilichiniCarl J Douglas
Jul 19, 2014·Science·Thomas MarcussenOdd-Arne Olsen
Jun 26, 2015·The New Phytologist·Philippa BorrillCristobal Uauy
Aug 28, 2015·Journal of Integrative Plant Biology·José Fernández GómezZoe A Wilson
Oct 8, 2015·Trends in Plant Science·Jianxin ShiDabing Zhang

❮ Previous
Next ❯

Citations

Jul 11, 2018·PloS One·Habibullah Khan AchakzaiAbdul Kabir Khan Achakzai
Jun 19, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Juan QiDaolin Fu
Apr 23, 2019·The Plant Journal : for Cell and Molecular Biology·Margaret A PallottaRyan Whitford
Dec 15, 2019·International Journal of Molecular Sciences·Junchang LiJishan Niu
Aug 8, 2021·International Journal of Molecular Sciences·Manjit SinghA Mark Cigan

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
confocal microscopy
scanning electron microscopy
genotyping
electrophoresis
Illumina sequencing

Software Mentioned

Sequencher
BLAST ( Basic Local Alignment Research Tool

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.