MsaB activates capsule production at the transcription level in Staphylococcus aureus

Microbiology
Justin L BatteMohamed O Elasri

Abstract

Staphylococcus aureus produces several virulence factors that allow it to cause a variety of infections. One of the major virulence factors is the capsule, which contributes to the survival of the pathogen within the host as a way to escape phagocytosis. The production of the capsular polysaccharide is encoded in a 16 gene operon, which is regulated in response to several environmental stimuli including nutrient availability. For instance, the capsule is produced in the late- and post-exponential growth phases, but not in the early- or mid-exponential growth phase. Several regulators are involved in capsule production, but the regulation of the cap operon is still poorly understood. In this study, we show that MsaB activates the cap operon by binding directly to a 10 bp repeat in the promoter region. We show that despite the fact that MsaB is expressed throughout four growth phases, it only activates capsule production in the late- and post-exponential growth phases. Furthermore, we find that MsaB does not bind to its target site in the early and mid-exponential growth phases. This correlates with decreased nutrient availability and capsule production. These data suggest either that MsaB binding ability changes in response to n...Continue Reading

References

Apr 1, 1984·Diagnostic Microbiology and Infectious Disease·R D ArbeitJ B Robbins
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Jan 27, 1999·FEMS Microbiology Letters·E R WannT J Foster
May 2, 2001·Genes & Development·M Ratnayake-LecamwasamA L Sonenshein
Jan 18, 2002·Infection and Immunity·Thanh LuongChia Y Lee
Jun 18, 2002·Infection and Immunity·Thanh T Luong, Chia Y Lee
Jun 19, 2003·Journal of Bacteriology·Thanh T LuongChia Y Lee
Jan 17, 2004·Clinical Microbiology Reviews·Katherine O'Riordan, Jean C Lee
Jan 22, 2004·FEMS Immunology and Medical Microbiology·Ambrose L CheungYan-Qiong Xiong
Apr 28, 2004·FEMS Microbiology Reviews·Stéphane BronnerGilles Prévost
Jun 19, 2004·Journal of Bacteriology·Markus BischoffSteven Projan
Jun 25, 2004·Nucleic Acids Research·Burkhard RostJinfeng Liu
Jul 2, 2004·Molecular Microbiology·Robert P Shivers, Abraham L Sonenshein
Oct 7, 2004·Applied and Environmental Microbiology·Emmanuelle CharpentierRichard P Novick
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jovanka M VoyichFrank R DeLeo
Jan 20, 2006·Molecular Microbiology·Jordan L CocchiaroJean C Lee
Apr 29, 2006·International Journal of Medical Microbiology : IJMM·Jan Pané-FarréMichael Hecker
Aug 21, 2008·Annual Review of Genetics·Richard P Novick, Edward Geisinger
Dec 18, 2008·BMC Microbiology·Karthik SambanthamoorthyMohamed O Elasri
Mar 3, 2009·Journal of Bacteriology·Konstanze PohlChristiane Wolz
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·Greg A Somerville, Richard A Proctor
Apr 7, 2010·Journal of Bacteriology·Charlotte D MajerczykAbraham L Sonenshein
May 27, 2011·The New England Journal of Medicine·Franklin D Lowy
May 11, 2012·Canadian Journal of Microbiology·Mrittika SenguptaRadheshyam K Jayaswal
Aug 25, 2012·Frontiers in Cellular and Infection Microbiology·Fabio BagnoliGuido Grandi
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Dec 20, 2012·Human Vaccines & Immunotherapeutics·Jasdeep S NanraAnnaliesa S Anderson

❮ Previous
Next ❯

Citations

Nov 24, 2017·BMC Microbiology·Gyan S SahukhalMohamed O Elasri
Jun 27, 2018·Journal of Bacteriology·Justin L BatteMohamed O Elasri
Jul 4, 2018·Journal of Bacteriology·Mei G Lei, Chia Y Lee
Jul 10, 2019·Molecular Microbiology·Daniela KeinhörsterChristiane Wolz
Aug 21, 2019·Journal of Bacteriology·Shanti PandeyMohamed O Elasri
Oct 21, 2020·Journal of Bacteriology·Mei G Lei, Chia Y Lee
Aug 1, 2019·International Journal of Medical Microbiology : IJMM·Daniela KeinhörsterChristiane Wolz
Apr 17, 2021·Current Microbiology·Basavaprabhu Haranahalli NatarajRashmi Hogarehalli Mallappa
Jun 3, 2021·Microorganisms·Francis MuchaambaTaurai Tasara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.