MSC/ECM Cellular Complexes Induce Periodontal Tissue Regeneration

Journal of Dental Research
Manabu TakewakiHidemi Kurihara

Abstract

Transplantation of mesenchymal stem cells (MSCs), which possess self-renewing properties and multipotency, into a periodontal defect is thought to be a useful option for periodontal tissue regeneration. However, developing more reliable and predictable implantation techniques is still needed. Recently, we generated clumps of an MSC/extracellular matrix (ECM) complex (C-MSC), which consisted of cells and self-produced ECM. C-MSCs can regulate their cellular functions in vitro and can be grafted into a defect site, without any artificial scaffold, to induce bone regeneration. Accordingly, this study aimed to evaluate the effect of C-MSC transplantation on periodontal tissue regeneration in beagle dogs. Seven beagle dogs were employed to generate a premolar class III furcation defect model. MSCs isolated from dog ilium were seeded at a density of 7.0 × 10(4) cells/well into 24-well plates and cultured in growth medium supplemented with 50 µg/mL ascorbic acid for 4 d. To obtain C-MSCs, confluent cells were scratched using a micropipette tip and were then torn off as a cellular sheet. The sheet was rolled up to make round clumps of cells. C-MSCs were maintained in growth medium or osteoinductive medium (OIM) for 5 or 10 d. The biolo...Continue Reading

References

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Citations

Jun 23, 2018·International Journal of Molecular Sciences·Fukushima TatsuhiroSatomura Kazuhito
Aug 28, 2018·Advanced Healthcare Materials·Cedryck VaquetteSaso Ivanovski
Feb 23, 2019·Journal of Cellular Physiology·Mudasir Bashir GugjooGutulla Taru Sharma
May 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Vidya N ChamundeswariSay Chye Joachim Loo
Sep 1, 2018·European Journal of Orthodontics·Yiping HuangWeiran Li
May 23, 2018·In Vitro Cellular & Developmental Biology. Animal·Yusuke AidaKoichi Kato
Aug 7, 2019·Current Opinion in Organ Transplantation·Andrea AngelettiPaolo Cravedi
Jun 11, 2020·Cell & Bioscience·Benson KohMuhammad Dain Yazid
Jan 28, 2021·Advanced Healthcare Materials·Wenkai JiaFeng Zhao
Oct 14, 2021·Tissue Engineering. Part B, Reviews·Yanjun GuanJiang Peng

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