Msp40 effector of root-knot nematode manipulates plant immunity to facilitate parasitism

Scientific Reports
Junhai NiuHeng Jian

Abstract

Root-knot nematodes (RKNs) are obligate biotrophic parasites that invade plant roots and engage in prolonged and intimate relationships with their hosts. Nematode secretions, some of which have immunosuppressing activity, play essential roles in successful parasitism; however, their mechanisms of action remain largely unknown. Here, we show that the RKN-specific gene MiMsp40, cloned from Meloidogyne incognita, is expressed exclusively in subventral oesophageal gland cells and is strongly upregulated during early parasitic stages. Arabidopsis plants overexpressing MiMsp40 were more susceptible to nematode infection than were wild type plants. Conversely, the host-derived MiMsp40 RNAi suppressed nematode parasitism and/or reproduction. Moreover, overexpression of MiMsp40 in plants suppressed the deposition of callose and the expression of marker genes for bacterial elicitor elf18-triggered immunity. Transient expression of MiMsp40 prevented Bax-triggered defence-related programmed cell death. Co-agroinfiltration assays indicated that MiMsp40 also suppressed macroscopic cell death triggered by MAPK cascades or by the ETI cognate elicitors R3a/Avr3a. Together, these results demonstrate that MiMsp40 is a novel Meloidogyne-specific e...Continue Reading

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Citations

Feb 9, 2018·International Journal of Molecular Sciences·Waqar IslamLiande Wang
Oct 20, 2017·Frontiers in Plant Science·Muhammad A AliHolger Bohlmann
Apr 5, 2018·Frontiers in Plant Science·Mateusz MatuszkiewiczMarcin Filipecki
Jun 6, 2018·International Journal of Molecular Sciences·Muhammad Amjad AliGyuhwa Chung
Jun 6, 2017·Frontiers in Plant Science·Sagar BanerjeeAnil Sirohi
Jan 21, 2021·Molecular Plant-microbe Interactions : MPMI·Sofia R CostaUlrike Mathesius
Nov 16, 2021·Planta·Maria E Lisei-de-SáMaria F Grossi-de-Sa

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Methods Mentioned

BETA
PCR
transgenic
PCRs

Software Mentioned

SPSS
SDS
Photoshop
Quantity One
Psort
Clustal X
MEGA
BLAST

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