Mst1 regulates post-infarction cardiac injury through the JNK-Drp1-mitochondrial fission pathway

Cellular & Molecular Biology Letters
Xisong Wang, Qing Song

Abstract

Post-infarction cardiac injury is closely associated with cardiac remodeling and heart dysfunction. Mammalian STE20-like kinase 1 (Mst1), a regulator of cellular apoptosis, is involved in cardiac remodeling in post-infarction heart, but the mechanisms remain poorly defined. We aimed to explore the role of Mst1 in regulating chronic post-infarction cardiac injury, with a focus on mitochondrial homoeostasis. Wild-type (WT) and Mst1-knockout mice were as the cardiac myocardial infarction model. Cardiac fibrosis, myocardial inflammation response, heart dysfunction and cardiomyocyte death were measured in vivo using immunohistochemistry, immunofluorescence, western blot, qPCR and TUNEL assays. Cardiomyocytes were isolated from WT and Mst1-knockout mice, and a chronic hypoxia model was used to induce damage. Mitochondrial function was determined via JC1 staining, ROS measurement, cyt-c leakage detection and mitochondrial apoptotic pathways analysis. Mitochondrial fission was observed using immunofluorescence. A pathway activator and inhibitor were applied to establish the signaling pathways involved in regulating mitochondrial homeostasis. Our study demonstrated that Mst1 expression was significantly upregulated in the heart post-inf...Continue Reading

References

Oct 26, 2016·Journal of Pineal Research·Jianqiang HuDongdong Sun
Nov 2, 2016·Journal of Pineal Research·Hai-Feng PeiYong-Jian Yang
Nov 28, 2016·Redox Biology·Gary B O'MealeyScott M Plafker
Dec 13, 2016·Angiogenesis·Elena Núñez-GómezJosé M López-Novoa
Dec 19, 2016·Angiogenesis·Timothy D Le CrasDenise M Adams
Dec 26, 2016·Basic Research in Cardiology·Voahanginirina RandriamboavonjyIngrid Fleming
Jan 7, 2017·Cell Death and Differentiation·Suruchi N SchockAstar Winoto
Jan 7, 2017·Cell Death and Differentiation·Daniella BrasacchioJoseph A Trapani
Jan 20, 2017·Basic Research in Cardiology·Carlotta RonchiAntonio Zaza
Jan 25, 2017·Basic Research in Cardiology·Nikolina JovancevicH Hatt
Feb 6, 2017·Basic Research in Cardiology·Wylly Ramsés García-NiñoCecilia Zazueta
Feb 12, 2017·Cell Death and Differentiation·Florent DufourOlivier Micheau
Feb 16, 2017·Angiogenesis·Janusz LigezaJolanta Jura
Feb 18, 2017·Cell Death and Differentiation·Jeanine L Van NostrandLaura D Attardi
Feb 22, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jing LiHaitao Wang
Jun 5, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Lu LiuXizheng Zhang
Jun 5, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Hong-Hui YangJian-Min Yao
Jun 5, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Wei ZhouZhaoxing Hu
Jun 12, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ning WangXiaoyuan Deng
Jul 4, 2017·Cellular Signalling·Nguyen Thi Kim OanhHyeseong Cho
Jul 18, 2017·Redox Biology·Maria LefakiNiki Chondrogianni

❮ Previous
Next ❯

Citations

Jun 20, 2019·Journal of Cellular Physiology·Xiuling ShangRongguo Yu
Mar 10, 2020·Clinical and Experimental Pharmacology & Physiology·Refaat A EidAttalla Farag El-Kott
Sep 5, 2019·Asian-Australasian Journal of Animal Sciences·Wei ZhangZongjun Yin
Sep 29, 2020·Frontiers in Cell and Developmental Biology·Ting XinFang Hu
Jul 21, 2020·Canadian Journal of Physiology and Pharmacology·Hao-Xi ZhaoYongsheng Yu
Apr 23, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Zhenyu XiongDongdong Sun
Aug 21, 2021·Frontiers in Physiology·Degang ChengChengzhi Lu

❮ Previous
Next ❯

Methods Mentioned

BETA
coronary artery bypass
ELISA
PCR
electron microscopy
confocal microscopy

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cardiotoxicity

Cardiotoxicity refers to the muscle damage or dysfunction of heart electrophysiology caused by drug intake or due to disease complications. It is a well-known side effect of several cytotoxic drugs, especially of the anthracyclines and can lead to long term morbidity. Here is the latest research.

Cardiac Remodeling

Cardiac remodeling in response to a myocardial infarction is characterized by progressive ventricular dilatation, cardiac hypertrophy, fibrosis, and deterioration of cardiac performance. Discover the latest research on Cardiac Remodeling here.