MST1 Suppression Reduces Early Brain Injury by Inhibiting the NF-κ B/MMP-9 Pathway after Subarachnoid Hemorrhage in Mice

Behavioural Neurology
Jie QuYujie Chen

Abstract

Mammalian sterile 20-like kinase 1 (MST1), the key component of the Hippo-YAP pathway, exhibits an important role in the pathophysiological process of various neurological disorders, including ischemic stroke and spinal cord injury. However, during subarachnoid hemorrhage, the involvement of MST1 in the pathophysiology of early brain injury remains unknown. We employed intravascular filament perforation to establish the subarachnoid hemorrhage (SAH) mouse model. The MST1 inhibitor XMU-MP-1 was intraperitoneally injected at 1 h after SAH, followed by daily injections. MST1 in vivo knockdown was performed 3 weeks prior to SAH via intracerebroventricular injection of adeno-associated virus (AAV) packaged with MST1 shRNA. The SAH grade, behavioral deficits, TUNEL staining, Evans blue dye extravasation and fluorescence, brain water content, protein and cytokine expressions by Western blotting, immunofluorescence, and proteome cytokine array were evaluated. Following SAH, the phosphorylation level of MST1 was upregulated at 12 h, with a peak at 72 h after SAH. It was colocalized with the microglial marker Iba1. Both XMU-MP-1 and MST1 shRNA alleviated the neurological deficits, blood-brain barrier (BBB) disruption, brain edema, neuroi...Continue Reading

References

Aug 24, 2004·Stroke; a Journal of Cerebral Circulation·S ParkJohn H Zhang
Jul 1, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lei XiaoZengqiang Yuan
Oct 17, 2012·Nature Reviews. Neurology·John H ZhangWilliam J Pearce
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jae Keun LeeEui-Ju Choi
Jul 31, 2013·Translational Stroke Research·Mutsumi FujiiJohn H Zhang
Oct 1, 2013·Progress in Neurobiology·Sheng ChenJohn H Zhang
Jan 24, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xingrong DuWufan Tao
May 24, 2014·PloS One·Konstantin V SalojinTamas Oravecz
Jan 1, 2015·Stroke; a Journal of Cerebral Circulation·Yujie ChenJohn H Zhang
Jun 28, 2015·Brain : a Journal of Neurology·Terrance T KummerDavid L Brody
Sep 17, 2015·Stroke; a Journal of Cerebral Circulation·Yusuke EgashiraGuohua Xi
May 31, 2016·Current Biology : CB·Sonali J RawatJonathan Chernoff
Jun 16, 2016·Circulation Journal : Official Journal of the Japanese Circulation Society·Shohei Ikeda, Junichi Sadoshima
Aug 19, 2016·Science Translational Medicine·Fuqin FanDawang Zhou
Sep 13, 2016·Biochemistry·Jacob A Galan, Joseph Avruch
Sep 28, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Budbazar EnkhjargalJohn H Zhang
Nov 2, 2016·The Journal of Clinical Investigation·Angela R M KurzMarkus Sperandio
Feb 2, 2017·Nature Communications·Chunxiao LiGuangwei Liu

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Citations

Nov 11, 2019·Journal of Neuroscience Research·Manas Ranjan Sahu, Amal Chandra Mondal
May 30, 2020·Genes, Brain, and Behavior·Yingchun ShangTao Zhang
May 24, 2019·International Journal of Molecular Sciences·Mehtab KhanMyeong Ok Kim
Mar 7, 2020·Frontiers in Immunology·Jinbo ChengZengqiang Yuan
Aug 25, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Linbang WangZhengxue Quan
Dec 5, 2020·Developmental Neurobiology·Manas Ranjan Sahu, Amal Chandra Mondal
Jul 18, 2020·Frontiers in Neuroscience·Jiayan JinYuan Gao
Apr 23, 2021·Biochemical Pharmacology·Zhigang ZhangYongsheng Yu
Oct 17, 2020·Neural Regeneration Research·Neha GogiaAmit Singh
Jun 3, 2021·International Journal of Molecular Sciences·Judith WeilandNadine Lilla
Jul 29, 2021·Immunity, Inflammation and Disease·Fang Zong, Yingzi Zhao
Dec 1, 2021·Expert Opinion on Therapeutic Targets·Ruchira M JhaJ Marc Simard

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