mTHPC-loaded extracellular vesicles outperform liposomal and free mTHPC formulations by an increased stability, drug delivery efficiency and cytotoxic effect in tridimensional model of tumors

Drug Delivery
Marie MillardLina Bezdetnaya

Abstract

Efficient photodynamic therapy with meta-tetra(hydroxyphenyl)chlorine requires the application of specific nanoformulations. mTHPC liposomal formulation (Foslip®) demonstrated favorable pharmacokinetics properties. However, rapid liposomes destruction in circulation and rapid mTHPC release impedes Foslip® applications. Alternatively, mTHPC nanovectorization using extracellular vesicles (EVs) could be an attractive option. EVs are naturally secreted by the organism to play a role in intercellular communication due to the capacity to transport proteins and nucleic acids. EVs also possess a natural ability to deliver therapeutic molecules into cancer cells. The aim of the present study was to evaluate photophysical and photobiological properties of mTHPC loaded in endothelial EVs as nanocarriers. We also studied efficiency of nanovectorisation on mTHPC distribution and PDT activity in multicellular tumor spheroids (MCTSs). MCTS is a nonvascularized in vitro 3D model of cells that mimics a similar microenvironment to in vivo situation. mTHPC-EVs were characterized by means of spectroscopic techniques, flow cytometry and nanoparticle tracking analysis. Compared with Foslip®, mTHPC-EVs are stable in murine plasma. Better mTHPC accumu...Continue Reading

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Citations

Jul 17, 2020·Physical Chemistry Chemical Physics : PCCP·Busenur AslanogluSaron Catak
Oct 18, 2019·Journal of Clinical Medicine·Ilya YakavetsLina Bezdetnaya
Jan 21, 2021·Cancers·Guillermo Bordanaba-FloritFélix Royo
Mar 23, 2021·Advanced Drug Delivery Reviews·Michaela Schulz-Siegmund, Achim Aigner
May 29, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ilya YakavetsLina Bezdetnaya
Jul 8, 2020·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ilya YakavetsLina Bezdetnaya
Oct 28, 2021·Advanced Healthcare Materials·Bryan Z WangGordana Vunjak-Novakovic

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Methods Mentioned

BETA
FCS
transmission electron microscopy
MDS
protein assay
confocal microscopy
flow cytometry
Fluorescence

Software Mentioned

LAS AF Life
NTA
LAS Life
ImageJ
Foslip

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