mTORC2 regulates hierarchical micro/nano topography-induced osteogenic differentiation via promoting cell adhesion and cytoskeletal polymerization.

Journal of Cellular and Molecular Medicine
Qian GaoLei Sui

Abstract

Surface topography acts as an irreplaceable role in the long-term success of intraosseous implants. In this study, we prepared the hierarchical micro/nano topography using selective laser melting combined with alkali heat treatment (SLM-AHT) and explored the underlying mechanism of SLM-AHT surface-elicited osteogenesis. Our results show that cells cultured on SLM-AHT surface possess the largest number of mature FAs and exhibit a cytoskeleton reorganization compared with control groups. SLM-AHT surface could also significantly upregulate the expression of the cell adhesion-related molecule p-FAK, the osteogenic differentiation-related molecules RUNX2 and OCN as well as the mTORC2 signalling pathway key molecule Rictor. Notably, after the knocked-down of Rictor, there were no longer significant differences in the gene expression levels of the cell adhesion-related molecules and osteogenic differentiation-related molecules among the three titanium surfaces, and the cells on SLM-AHT surface failed to trigger cytoskeleton reorganization. In conclusion, the results suggest that mTORC2 can regulate the hierarchical micro/nano topography-mediated osteogenesis via cell adhesion and cytoskeletal reorganization.

References

Jul 29, 1998·Medical Engineering & Physics·J Y RhoP Zioupos
Oct 7, 2004·Nature Cell Biology·Estela JacintoMichael N Hall
Jul 27, 2005·The Journal of Biological Chemistry·Tripti GaurJane B Lian
Sep 7, 2005·Biomaterials·Matthew John DalbyRichard O C Oreffo
Dec 29, 2007·Cell Communication & Adhesion·Sepideh Heydarkhan-HagvallRamin E Beygui
Nov 1, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Paulo G CoelhoJack E Lemons
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Benjamin GeigerAlexander D Bershadsky
Oct 11, 2011·Critical Reviews in Biochemistry and Molecular Biology·Bing Su, Estela Jacinto
Apr 17, 2012·Cell·Mathieu Laplante, David M Sabatini
Jan 15, 2013·Biomaterials·Penelope M TsimbouriMatthew J Dalby
Jul 4, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Buer SenJanet Rubin
Jan 5, 2014·The Journal of Cell Biology·Charles Betz, Michael N Hall
Feb 1, 2014·Breast Cancer Research : BCR·Manish TandonJitesh Pratap
Feb 28, 2014·Cell Biochemistry and Function·Nirali ShahRichard Manasseh
May 23, 2014·Nature Materials·Matthew J DalbyRichard O C Oreffo
Sep 10, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jianquan ChenFanxin Long
Apr 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yu ShiFanxin Long
Jun 20, 2015·PloS One·Jianquan Chen, Fanxin Long
Aug 5, 2015·Journal of Cell Science·Vera AuernheimerWolfgang H Goldmann
Feb 26, 2016·Cell Biology International·Wolfgang H Goldmann
Jun 6, 2016·Bone·Dong-Mei LiuJian-Min Liu
Oct 14, 2016·Cell Death & Disease·Pinling LaiXiaochun Bai
Mar 12, 2017·Cell·Robert A Saxton, David M Sabatini
Aug 30, 2018·Journal of Proteome Research·Hao WangRuibing Chen
Nov 6, 2018·Cellular and Molecular Life Sciences : CMLS·Jiayun HuangWeiliang Shen
Nov 18, 2018·Acta Biomaterialia·Furqan A ShahAnders Palmquist
Dec 20, 2018·Frontiers in Bioengineering and Biotechnology·Chiara CimminoMaurizio Ventre
Mar 10, 2019·Biochemical and Biophysical Research Communications·Yongyun ChangHuiwu Li
May 28, 2019·Acta Biomaterialia·Julio C M SouzaLyndon F Cooper
Aug 6, 2019·Frontiers in Bioengineering and Biotechnology·Takao Hanawa
Aug 21, 2019·International Journal of Molecular Sciences·Juri SarutaTakahiro Ogawa

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