Mu-opioid receptor down-regulation and tolerance are not equally dependent upon G-protein signaling

Pharmacology, Biochemistry, and Behavior
B A GomesB C Yoburn

Abstract

In the present study, the contribution of pertussis toxin (PTX)-sensitive G(i/o)-proteins to opioid tolerance and mu-opioid receptor down-regulation in the mouse were examined. Mice were injected once intracerebroventricularly and intrathecally with PTX (0.1 microg/site). Controls were treated with saline. On the 10th day following PTX treatment, continuous subcutaneous infusion of etorphine (150 or 200 microg/kg/day) or morphine (40 mg/kg/day+25 mg slow-release pellet) was begun. Control mice were implanted with inert placebo pellets. Pumps and pellets were removed 3 days later, and mice were tested for morphine analgesia or mu-opioid receptor density was determined in the whole brain, spinal cord, and midbrain. Both infusion doses of etorphine produced significant tolerance (ED50 shift=approximately 4-6-fold) and down-regulation of mu-opioid receptors (approximately 20-35%). Morphine treatment also produced significant tolerance (ED50 shift= approximately 5-8-fold), but no mu-opioid receptor down-regulation. PTX dramatically reduced the acute potency of morphine and blocked the further development of tolerance by both etorphine and morphine treatments. However, PTX had no effect on etorphine-induced mu-opioid receptor down-re...Continue Reading

References

Apr 1, 1991·Pharmacology, Biochemistry, and Behavior·S C ChangB C Yoburn
May 15, 1990·Biochemical Pharmacology·T Reisine
Jan 1, 1990·Pharmacology, Biochemistry, and Behavior·D ParolaroM Parenti
May 1, 1995·Anesthesiology·A Duttaroy, B C Yoburn
Jun 2, 1994·European Journal of Pharmacology·R B RaffaC D Connelly
Aug 9, 1996·The Journal of Biological Chemistry·D E KeithM von Zastrow
Mar 1, 1997·Pharmacology, Biochemistry, and Behavior·S ShahB C Yoburn
Sep 1, 1997·Pharmacology, Biochemistry, and Behavior·A DuttaroyB C Yoburn
Nov 20, 1997·The Journal of Biological Chemistry·Y YuJ B Wang
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J ZhangM G Caron
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·J L Whistler, M von Zastrow
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·P Y LawH H Loh
Jun 23, 2001·Pharmacology, Biochemistry, and Behavior·K StaffordB C Yoburn

❮ Previous
Next ❯

Citations

Nov 13, 2003·Peptides·Richard J Bodnar, Maria M Hadjimarkou
Mar 22, 2008·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Shahid Husain, David E Potter
Apr 21, 2010·Pediatrics·Kanwaljeet J S AnandUNKNOWN Eunice Kennedy Shriver National Institute of Child Health and Human Development Collaborative Pediatric Critical Care Resear
Dec 14, 2007·Pharmacology & Therapeutics·Thomas Koch, Volker Höllt
Jan 8, 2015·Frontiers in Pharmacology·Stéphane AlloucheNicolas Marie
Apr 16, 2019·The Clinical Journal of Pain·Julia C Finkel, Jennifer Pukish
Nov 19, 2002·Molecular Pharmacology·Minesh B PatelByron C Yoburn
Mar 23, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sabine SpijkerAugust B Smit
Jun 26, 2012·British Journal of Pharmacology·Christian DollStefan Schulz
Oct 18, 2018·Science Signaling·Ben HitchinsonVadim Gaponenko

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.