MUC16 impacts tumor proliferation and migration through cytoplasmic translocation of P120-catenin in epithelial ovarian cancer cells: an original research

BMC Cancer
Xin ChenXipeng Wang

Abstract

Epithelial ovarian cancer (EOC) remains one of the most lethal gynecologic cancers, and its pathogenetic mechanism remains unclear. Here we show that MUC16 promotes the translocation of p120-catenin (p120ctn) to the cytoplasm and consequently activates ras homolog (Rho) GTPases RhoA/Cdc42 activation to modulate the proliferation and migration abilities of EOC cells. We collect 94 ovarian cancer (OC) patients' tissue samples to constitute tissue microarray (TMA) and analyze the MUC16 and p120ctn expression levels. Lentivirus transfection is used to overexpress cytoplasmic tail domain (CTD) of MUC16 and CRISPR/Cas9 genome-editing system is firstly used to knock out MUC16 in EOC cells. The proliferation or migration ability of cells is analyzed by MTS or migration assay. We find that MUC16 and p120ctn are aberrantly overexpressed in 94 clinical OC samples compared with benign ovarian tumors (BOT). MUC16 is a critical inducer of the proliferation and migration of EOC cells and the CTD of MUC16 plays an important role during this process. In addition, we reveal the relationship between MUC16 and p120ctn, which has not previously been studied. We show that MUC16 promotes the translocation of p120ctn to the cytoplasm and consequently ...Continue Reading

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Citations

Feb 11, 2020·Clinical Genetics·Wei ZhangHongbo Wang
Feb 6, 2021·Journal of Cellular and Molecular Medicine·Fang WangJinquan Cui
Jan 10, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Minghai ZhangYu Wang
Feb 7, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David J BirnbaumAndrew S Liss
May 14, 2021·Digestive Diseases and Sciences·Zhining LiuQiang Huang
Nov 12, 2021·Cancer Investigation·Shuang LuoHailiang Song

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Methods Mentioned

BETA
GTPases
GTPase
pull-down
PCR

Software Mentioned

SPSS
ImageJ

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