Abstract
Neoplastic transformation of epithelial cells is commonly associated with alterations in the expression of mucin genes. The mechanisms involved in this process are largely unknown. MUC6, isolated from a stomach cDNA library, is mainly expressed in stomach antral glands, as detected by using in situ hybridization and immunohistochemistry. We examined MUC6 expression in normal and pathological breast tissues using immunohistochemistry with MUC6-specific antibodies and in cultured breast cancer cells using immunocytochemistry and Northern blotting. MUC6 was generally not detected in normal breast (1/11) but was detected in fibrocystic disease without atypia (7/17, 41%), in atypical fibrocystic disease (11/11, 100%) and in carcinoma (57/60, 95%). To study the mechanisms involved in mucin gene up-regulation in breast cancer, we examined baseline, growth-related and steroid-induced levels of MUC1, MUC3 and MUC6 in 4 breast cancer cell lines, 2 of which express estrogen receptors. MUC6 levels were up-regulated at post-confluence in 2/4 cell lines, whereas no changes were detected for the other mucin genes examined. MUC6 and MUC3 were constitutively expressed, and steroid-induced, in BT-474 and MCF-7 cells, respectively. As a control, ...Continue Reading
References
Dec 1, 1991·Molecular Endocrinology·T SaekiD S Salomon
Jul 1, 1991·The American Journal of Surgical Pathology·D E Haagensen
Nov 1, 1990·Cancer Genetics and Cytogenetics·P R KadamS H Advani
Aug 5, 1988·Science·M C RioP Chambon
Dec 20, 1982·Nucleic Acids Research·P MasiakowskiP Chambon
Dec 16, 1993·Nature·Y Shimizu, S Shaw
Sep 1, 1995·Gastroenterology·C De BolósF X Real
Jan 1, 1993·Gastroenterology·G GambúsF X Real
Apr 1, 1995·Human Pathology·M A McGuckinR G Wright
Jan 1, 1995·Human Reproduction·J P AudieA Boersma
Jun 9, 1995·The Journal of Biological Chemistry·T LesuffleurF X Real
Jan 1, 1995·Annual Review of Physiology·S J Gendler, A P Spicer
Jan 1, 1994·Annual Review of Biochemistry·M J Tsai, B W O'Malley
Jul 1, 1994·Gastroenterology·C CarratoF X Real
Jul 1, 1994·Gastroenterology·S K ChangY S Kim
Jul 15, 1994·Biochemical and Biophysical Research Communications·S TsukadaT Kageyama
Jan 1, 1994·Breast Cancer Research and Treatment·Y S López-BoadoC López-Otín
Mar 1, 1994·Digestive Diseases and Sciences·L PolimenoA Francavilla
Apr 1, 1994·Gastroenterology·C BalaguéF X Real
Oct 1, 1993·Journal of Clinical Pathology·M D WalshR G Wright
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M Abe, D Kufe
Dec 26, 1995·Biochemical and Biophysical Research Communications·E G GollubZ Marom
Mar 15, 1996·The Journal of Biological Chemistry·K B CleutjensJ Trapman
Jun 21, 1996·The Journal of Biological Chemistry·M Balbín, C López-Otín
Sep 1, 1995·Journal of Gastroenterology and Hepatology·V ApostolopoulosI F McKenzie
Citations
Mar 17, 2001·Journal of Clinical Pathology·M B PereiraF C Schmitt
Feb 2, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Michael D WalshDaniel D Buchanan
Jan 12, 2001·American Journal of Respiratory Cell and Molecular Biology·A López-Ferrer de Bolós C
May 16, 2002·Histopathology·X LeroyN Porchet
Dec 13, 2002·International Journal of Cancer. Journal International Du Cancer·Nora BeroisEduardo Osinaga
May 19, 2012·Journal of Comparative Pathology·C TavaresF Gärtner
Aug 20, 2011·Experimental Cell Research·Shih-Hsing Leir, Ann Harris
Mar 30, 2010·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Kimihide KusafukaTakashi Nakajima
Apr 25, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A Martínez-AntónJ Mullol
Feb 1, 2011·Biochimica Et Biophysica Acta·Partha MukhopadhyaySurinder K Batra
May 7, 2016·Biology Direct·Bálint MészárosZsuzsanna Dosztányi
Sep 9, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Cecilia SóñoraEduardo Osinaga
Oct 26, 1999·Human Pathology·F C SchmittC A Reis
Jun 25, 2005·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Emad A RakhaIan O Ellis
Feb 15, 2001·Journal of Cellular Physiology·P LiK L Carraway
May 18, 2005·The American Journal of Surgical Pathology·Llucia AlosAntonio Cardesa
Sep 22, 2010·Medical Molecular Morphology·Kimihide KusafukaTakashi Nakajima
Dec 3, 2005·Virchows Archiv : an International Journal of Pathology·Kukka HeiskalaLeif C Andersson
Mar 21, 2012·Medical Molecular Morphology·Kimihide KusafukaTakashi Nakajima
Apr 23, 2013·Cancer Immunology, Immunotherapy : CII·Daniel MazalEduardo Osinaga
Jul 29, 2003·The American Journal of Surgical Pathology·Roderick H W SimpsonLeonor David
Jul 17, 2004·The International Journal of Biological Markers·M QuarantaF Schittulli
Sep 6, 2005·Cancer Research·Teresa FreireEduardo Osinaga
May 2, 2000·Critical Reviews in Oncology/hematology·K A Carolin, H A Pass