Abstract
The expression pattern of mucin genes was studied in 7 lung adenocarcinoma cell lines (CL1, CL2, CL3, NCL2, PC9, PC13, PC14) and 12 lung adenocarcinoma tissues. CL1 and PC13 are poorly differentiated cell lines with low mucin glycoprotein production. The other 5 cell lines are well differentiated and produce a higher amount of mucins. Total RNA was extracted from these cell lines. Northern blot analysis was performed by hybridization with specific antisense oligonucleotide probes recognizing mucin-specific tandem repeats of 4 mucin genes (MUC1, MUC2, MUC3, MUC4). RT-PCR was carried out to amplify the 3' and 5' nonrepetitive coding regions of MUC1 and the 5' nonrepetitive coding region of MUC2. All these cell lines expressed MUC1, MUC2, and MUC4 mRNA but in variable mounts. The poorly differentiated cell lines (CL1 and PC13) had a relatively low level of expression of MUC1, MUC2, MUC3 and MUC4. RT-PCR, with primers amplifying the MUC1 nonrepetitive coding region 5' end, 293 bp, and the 3' end, 522 bp, as well as the MUC2 nonrepetitive 5' coding region, 308 bp, revealed the presence of MUC1 and MUC2 mRNA in all the cell lines. Sequence analysis of the PCR products were very homologous, similar to previously published MUC1 and MUC...Continue Reading
Citations
Oct 12, 2001·Cancer·J HanaokaI Ohkubo
Oct 26, 1999·Cancer Letters·M StimpflR Zeillinger
May 18, 2005·The American Journal of Surgical Pathology·Llucia AlosAntonio Cardesa
Jan 28, 1999·American Journal of Respiratory Cell and Molecular Biology·M P BuisineN Porchet
Feb 25, 1999·American Journal of Respiratory Cell and Molecular Biology·J T BergerM C Rose
Sep 29, 2005·BMC Cancer·Vidya HebbarGoverdhan P Sachdev
Nov 19, 2011·PloS One·Rong LuJian Ge
Aug 1, 2008·Expert Opinion on Medical Diagnostics·Subhankar ChakrabortySurinder K Batra
Dec 22, 1999·European Journal of Cell Biology·M CornbergS Wagner
May 7, 2013·Bioconjugate Chemistry·André A NevesKevin M Brindle
Jul 31, 2004·The Laryngoscope·Donald T WeedKermit L Carraway
Nov 28, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Hideaki TsutsumidaSuguru Yonezawa
Jul 4, 2006·Pathology, Research and Practice·Aydanur KargAhmet Uçvet
Apr 24, 1999·The Annals of Thoracic Surgery·A OhgamiK Yasumoto
Mar 29, 2001·Human Pathology·M C CopinN Porchet
Apr 16, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·André A NevesKevin M Brindle
Jan 11, 2018·Scientific Reports·Amany Sayed MawasYukio Takeshima
Mar 30, 2000·International Journal of Cancer. Journal International Du Cancer·M C CopinN Porchet
Nov 28, 2018·Oncotarget·Andreas SaltosJhanelle E Gray
Apr 12, 2007·Archives of Pathology & Laboratory Medicine·Kun Y KwonPhilip T Cagle