Mucin mRNA expression in lung adenocarcinoma cell lines and tissues

Oncology
C J YuC W Wu

Abstract

The expression pattern of mucin genes was studied in 7 lung adenocarcinoma cell lines (CL1, CL2, CL3, NCL2, PC9, PC13, PC14) and 12 lung adenocarcinoma tissues. CL1 and PC13 are poorly differentiated cell lines with low mucin glycoprotein production. The other 5 cell lines are well differentiated and produce a higher amount of mucins. Total RNA was extracted from these cell lines. Northern blot analysis was performed by hybridization with specific antisense oligonucleotide probes recognizing mucin-specific tandem repeats of 4 mucin genes (MUC1, MUC2, MUC3, MUC4). RT-PCR was carried out to amplify the 3' and 5' nonrepetitive coding regions of MUC1 and the 5' nonrepetitive coding region of MUC2. All these cell lines expressed MUC1, MUC2, and MUC4 mRNA but in variable mounts. The poorly differentiated cell lines (CL1 and PC13) had a relatively low level of expression of MUC1, MUC2, MUC3 and MUC4. RT-PCR, with primers amplifying the MUC1 nonrepetitive coding region 5' end, 293 bp, and the 3' end, 522 bp, as well as the MUC2 nonrepetitive 5' coding region, 308 bp, revealed the presence of MUC1 and MUC2 mRNA in all the cell lines. Sequence analysis of the PCR products were very homologous, similar to previously published MUC1 and MUC...Continue Reading

Citations

Oct 26, 1999·Cancer Letters·M StimpflR Zeillinger
Jan 28, 1999·American Journal of Respiratory Cell and Molecular Biology·M P BuisineN Porchet
Feb 25, 1999·American Journal of Respiratory Cell and Molecular Biology·J T BergerM C Rose
Sep 29, 2005·BMC Cancer·Vidya HebbarGoverdhan P Sachdev
Aug 1, 2008·Expert Opinion on Medical Diagnostics·Subhankar ChakrabortySurinder K Batra
May 7, 2013·Bioconjugate Chemistry·André A NevesKevin M Brindle
Nov 28, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Hideaki TsutsumidaSuguru Yonezawa
Apr 16, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·André A NevesKevin M Brindle
Mar 30, 2000·International Journal of Cancer. Journal International Du Cancer·M C CopinN Porchet
Nov 28, 2018·Oncotarget·Andreas SaltosJhanelle E Gray
Apr 12, 2007·Archives of Pathology & Laboratory Medicine·Kun Y KwonPhilip T Cagle

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